CYLD, Polyclonal Antibody

Anti-CYLD Antibody (Internal) IHC-plus; CYLD; CDMT; Deubiquitinating enzyme CYLD; CYLDI; EAC; HSPC057; MFT1; MFT; SBS; TEM; BRSS; Ubiquitin thiolesterase CYLD; USPL2; CYLD1; KIAA0849; Ubiquitin thioesterase CYLD; Mouse CYLD

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Goat

Mouse CYLD.

Immunoaffinity Purified

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide

0.5 mg/ml (lot specific)

Store at -20 degree C. Minimize freezing and thawing.

Small volumes of anti-CYLD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)

ELISA (1:32000), IHC-P (5 ug/ml), WB (1:32000, 0.5 - 1.5 ug/ml)

Anti-CYLD antibody IHC of mouse brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Antibody (0.5 ug/ml) staining of rat brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

107,044 Da

cylindromatosis (turban tumor syndrome)

ubiquitin carboxyl-terminal hydrolase CYLD; ubiquitin thioesterase CYLD; deubiquitinating enzyme CYLD; ubiquitin thiolesterase CYLD; ubiquitin specific peptidase like 2; ubiquitin-specific-processing protease CYLD; probable ubiquitin carboxyl-terminal hydrolase CYLD

Ubiquitin carboxyl-terminal hydrolase CYLD

CYLD1; KIAA0849  [Similar Products]

CYLD_HUMAN

This gene is encodes a cytoplasmic protein with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains that functions as a deubiquitinating enzyme. Mutations in this gene have been associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

CYLD: Protease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Has endodeubiquitinase activity. Plays an important role in the regulation of pathways leading to NF-kappa-B activation. Contributes to the regulation of cell survival, proliferation and differentiation via its effects on NF-kappa-B activation. Negative regulator of Wnt signaling. Inhibits HDAC6 and thereby promotes acetylation of alpha-tubulin and stabilization of microtubules. Plays a role in the regulation of microtubule dynamics, and thereby contributes to the regulation of cell proliferation, cell polarization, cell migration, and angiogenesis. Required for normal cell cycle progress and normal cytokinesis. Inhibits nuclear translocation of NF-kappa-B. Plays a role in the regulation of inflammation and the innate immune response, via its effects on NF-kappa-B activation. Dispensable for the maturation of intrathymic natural killer cells, but required for the continued survival of immature natural killer cells. Negatively regulates TNFRSF11A signaling and osteoclastogenesis. Interacts (via CAP-Gly domain) with IKBKG/NEMO (via proline-rich C-terminal region). Interacts with TRAF2 and TRIP. Interacts with PLK1, DVL1, DVL3, MAVS, TBK1, IKKE and DDX58. Interacts (via CAP-Gly domain) with microtubules. Interacts with HDAC6 and BCL3. Interacts with SQSTM1 and MAP3K7. Identified in a complex with TRAF6 and SQSTM1. Detected in fetal brain, testis, and skeletal muscle, and at a lower level in ***** brain, leukocytes, liver, heart, kidney, spleen, ovary and lung. Isoform 2 is found in all tissues except kidney. Inhibited by phosphorylation at serine residues. Belongs to the peptidase C67 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin-specific protease; EC 3.4.19.12; Ubiquitin conjugating system; Protease; Tumor suppressor

Chromosomal Location of Human Ortholog: 16q12.1

Cellular Component: nucleoplasm; centrosome; extrinsic to internal side of plasma membrane; perinuclear region of cytoplasm; cytoplasmic microtubule; spindle; midbody; cytosol

Molecular Function: protein binding; structural constituent of ribosome; zinc ion binding; ubiquitin-specific protease activity; protein kinase binding

Biological Process: ubiquitin-dependent protein catabolic process; Wnt receptor signaling pathway; inhibition of NF-kappaB transcription factor; regulation of mitotic cell cycle; innate immune response; negative regulation of NF-kappaB import into nucleus; cell cycle; negative regulation of interferon type I production; negative regulation of T cell differentiation

Disease: Brooke-spiegler Syndrome; Cylindromatosis, Familial; Trichoepithelioma, Multiple Familial, 1

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