Full Product Name
RPA30 Antibody
Product Synonym Names
RPA4; HSU24186; Replication protein A4; 30kDa; RF-A protein 4; RP-A p30; Replication protein A4; 34kDa; Replication factor A protein 4
Product Gene Name
anti-RPA30 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q13156
Specificity
RPA30 Antibody detects endogenous levels of total RPA30
Form/Format
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0mg/mL (lot specific)
Immunogen
A synthesized peptide derived from human RPA30
Preparation and Storage
Store at -20 degree C
Other Notes
Small volumes of anti-RPA30 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-RPA30 antibody
Total protein Ab
Applications Tested/Suitable for anti-RPA30 antibody
Western Blot (WB)
Application Notes for anti-RPA30 antibody
Western Blot: 1:1000-3000
Western Blot (WB) of anti-RPA30 antibody
Western blot analysis RPA30 using COLO205 whole cell lysates
NCBI/Uniprot data below describe general gene information for RPA30. It may not necessarily be applicable to this product.
NCBI Accession #
NP_037479.1
[Other Products]
NCBI GenBank Nucleotide #
NM_013347.4
[Other Products]
UniProt Primary Accession #
Q13156
[Other Products]
UniProt Secondary Accession #
Q3SY03[Other Products]
UniProt Related Accession #
Q13156[Other Products]
Molecular Weight
28,868 Da
NCBI Official Full Name
replication protein A 30 kDa subunit
NCBI Official Synonym Full Names
replication protein A4
NCBI Official Symbol
RPA4 [Similar Products]
NCBI Official Synonym Symbols
HSU24186
[Similar Products]
NCBI Protein Information
replication protein A 30 kDa subunit
UniProt Protein Name
Replication protein A 30 kDa subunit
UniProt Synonym Protein Names
Replication factor A protein 4; RF-A protein 4
UniProt Gene Name
RPA4 [Similar Products]
UniProt Synonym Gene Names
RP-A p30; RF-A protein 4 [Similar Products]
UniProt Entry Name
RFA4_HUMAN
NCBI Summary for RPA30
This gene encodes a single-stranded DNA-binding protein that is the 30-kDa subunit of the replication protein A complex. Replication protein A is an essential factor for DNA double-strand break repair and cell cycle checkpoint activation. The encoded protein localizes to DNA repair foci and may be involved in the cellular DNA damage response. This protein may also play a role in inhibiting viral replication.[provided by RefSeq, Apr 2010]
UniProt Comments for RPA30
RPA4: functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange. Component of the alternative replication protein A complex (aRPA) composed of RPA1, RPA3 and RPA4. Interacts with RPA1 and RPA3. Localizes to DNA repair foci after DNA damage.
Chromosomal Location of Human Ortholog: Xq21.33
Cellular Component: DNA replication factor A complex; nucleoplasm; nucleus
Molecular Function: single-stranded DNA binding
Biological Process: DNA damage checkpoint; DNA replication during S phase; DNA replication initiation; G1/S transition of mitotic cell cycle; nucleotide-excision repair
Research Articles on RPA30
1. Findings provide the first direct evidence for the function of alternative form of RPA in human DNA metabolism and support a model for aRPA functioning in chromosome maintenance functions in nonproliferating cells.
Precautions
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Disclaimer
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