alpha Synuclein, ELISA Kit

Canine a Synuclein ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of alpha-SYN. No significant cross-reactivity or interference between alpha-SYN and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between alpha-SYN and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of alpha-SYN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS739584 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the alpha Synuclein (alpha-SYN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing alpha-SYN. The ELISA analytical biochemical technique of the MBS739584 kit is based on alpha-SYN antibody-alpha-SYN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect alpha-SYN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, alpha-SYN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the assay: alpha-SYN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-alpha-SYN antibody and an alpha-SYN-HRP conjugate. The assay sample and buffer are incubated together with alpha-SYN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the alpha-SYN concentration since alpha-SYN from samples and alpha-SYN-HRP conjugate compete for the anti-alpha-SYN antibody . Since the number of sites is limited, as more sites are occupied by alpha-SYN from the sample, fewer sites are left to bind alpha-SYN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The alpha-SYN concentration in each sample is interpolated from this standard curve.

Neurobiology

12,345 Da

synuclein, alpha

alpha-synuclein; non-A4 component of amyloid; non-A beta component of AD amyloid

Alpha-synuclein

Syn; NACP  [Similar Products]

SYUA_MOUSE

SNCA: a member of the synuclein family. Abundantly expressed in the brain. Inhibits phospholipase D2 selectively. May integrate presynaptic signaling and membrane trafficking. Implicated in the pathogenesis of Parkinson's disease. A major component of amyloid plaques in the brains of patients with Alzheimer's disease. Two alternatively spliced isoforms transcripts have been identified.

Protein type: Adaptor/scaffold

Cellular Component: Golgi apparatus; nuclear outer membrane; cytoplasmic vesicle membrane; intracellular membrane-bound organelle; rough endoplasmic reticulum; mitochondrion; cell; fibril; terminal button; inclusion body; cell cortex; cytosol; actin cytoskeleton; platelet alpha granule membrane; synaptic vesicle; cytoskeleton; growth cone; axon; perinuclear region of cytoplasm; cytoplasm; plasma membrane; ribosome; synapse; nerve terminal; nucleus

Molecular Function: identical protein binding; histone binding; zinc ion binding; kinesin binding; ferrous iron binding; metal ion binding; magnesium ion binding; Hsp70 protein binding; oxidoreductase activity; calcium ion binding; enzyme binding; phospholipase binding; phospholipid binding; alpha-tubulin binding; protein domain specific binding; microtubule binding; caspase inhibitor activity; beta-tubulin binding; phosphoprotein binding; protein N-terminus binding; arachidonic acid binding; dynein binding; protein binding; copper ion binding; tau protein binding

Biological Process: regulation of acyl-CoA biosynthetic process; ***** locomotory behavior; regulation of neurotransmitter secretion; positive regulation of endocytosis; response to lipopolysaccharide; dopamine biosynthetic process; positive regulation of neurotransmitter secretion; dopamine metabolic process; response to magnesium ion; regulation of neuron apoptosis; synapse organization and biogenesis; negative regulation of neuron apoptosis; response to drug; negative regulation of dopamine metabolic process; synaptic vesicle endocytosis; negative regulation of transporter activity; regulation of neuronal synaptic plasticity; negative regulation of apoptosis; regulation of long-term neuronal synaptic plasticity; negative regulation of serotonin uptake; synaptic transmission, dopaminergic; negative regulation of norepinephrine uptake; mitochondrial membrane organization and biogenesis; microglial cell activation; negative regulation of monooxygenase activity; negative regulation of caspase activity; fatty acid metabolic process; regulation of dopamine secretion; positive regulation of synaptic transmission; negative regulation of dopamine uptake; negative regulation of histone acetylation; synaptic transmission; negative regulation of exocytosis; negative regulation of protein amino acid phosphorylation; behavioral response to cocaine; receptor internalization; phospholipid metabolic process; response to iron(II) ion; positive regulation of receptor recycling; caspase activation; neutral lipid metabolic process; protein destabilization; regulation of macrophage activation; negative regulation of microtubule polymerization; regulation of glutamate secretion; synaptic vesicle transport; positive regulation of peptidyl-serine phosphorylation; organelle ATP synthesis coupled electron transport; regulation of locomotion; positive regulation of release of sequestered calcium ion into cytosol; regulation of excitatory postsynaptic membrane potential

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