Full Product Name
RPA4 antibody - middle region
Product Gene Name
anti-RPA4 antibody
[Similar Products]
Product Synonym Gene Name
HSU24186; MGC120333; MGC120334[Similar Products]
Antibody/Peptide Pairs
RPA4 peptide (MBS3236902) is used for blocking the activity of RPA4 antibody (MBS3211955)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
Synthetic peptide located within the following region: VPVSPSEVND AGDNDESHRN FIQDEVLRLI HECPHQEGKS IHELRAQLCD
3D Structure
ModBase 3D Structure for Q13156
Purity/Purification
Affinity Purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Immunogen
The immunogen is a synthetic peptide directed towards the middle region of human RPA4
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-RPA4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-RPA4 antibody
This is a rabbit polyclonal antibody against RPA4. It was validated on Western Blot using a cell lysate as a positive control.
Target Description: Replication protein A (RPA) is an essential factor for DNA double-strand break repair and cell cycle checkpoint activation. RPA4 is the 32-kDa subunit of the RPA, which associates with the 70- and 13-kDa subunits to form a trimeric RPA complex. Replication protein A (RPA) is an essential factor for DNA double-strand break repair and cell cycle checkpoint activation. This gene encodes the 32-kDa subunit of the RPA, which associates with the 70- and 13-kDa subunits to form a trimeric RPA complex. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-1448 BC069824.1 1-1448
Product Categories/Family for anti-RPA4 antibody
Polyclonal; Cell Cycle Proteins; DNA Damage & Repair; Meiosis/Mitosis/Cell Cycle;
Applications Tested/Suitable for anti-RPA4 antibody
Western Blot (WB)
Western Blot (WB) of anti-RPA4 antibody
WB Suggested Anti-RPA4 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:2500
Positive Control: Hela cell lysate
NCBI/Uniprot data below describe general gene information for RPA4. It may not necessarily be applicable to this product.
NCBI Accession #
NP_037479
[Other Products]
NCBI GenBank Nucleotide #
NM_013347
[Other Products]
UniProt Primary Accession #
Q13156
[Other Products]
UniProt Related Accession #
Q13156[Other Products]
NCBI Official Full Name
replication protein A 30 kDa subunit
NCBI Official Synonym Full Names
replication protein A4
NCBI Official Symbol
RPA4 [Similar Products]
NCBI Official Synonym Symbols
HSU24186
[Similar Products]
NCBI Protein Information
replication protein A 30 kDa subunit
UniProt Protein Name
Replication protein A 30 kDa subunit
UniProt Synonym Protein Names
Replication factor A protein 4
Protein Family
Replication protein
UniProt Gene Name
RPA4 [Similar Products]
UniProt Synonym Gene Names
RP-A p30 [Similar Products]
UniProt Entry Name
RFA4_HUMAN
NCBI Summary for RPA4
This gene encodes a single-stranded DNA-binding protein that is the 30-kDa subunit of the replication protein A complex. Replication protein A is an essential factor for DNA double-strand break repair and cell cycle checkpoint activation. The encoded protein localizes to DNA repair foci and may be involved in the cellular DNA damage response. This protein may also play a role in inhibiting viral replication.[provided by RefSeq, Apr 2010]
UniProt Comments for RPA4
RPA4: functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange. Component of the alternative replication protein A complex (aRPA) composed of RPA1, RPA3 and RPA4. Interacts with RPA1 and RPA3. Localizes to DNA repair foci after DNA damage.
Chromosomal Location of Human Ortholog: Xq21.33
Cellular Component: nucleoplasm; DNA replication factor A complex; nucleus
Molecular Function: single-stranded DNA binding
Biological Process: DNA replication initiation; nucleotide-excision repair; DNA damage checkpoint; DNA replication during S phase; mitotic cell cycle; G1/S transition of mitotic cell cycle; DNA recombination
Research Articles on RPA4
1. Findings provide the first direct evidence for the function of alternative form of RPA in human DNA metabolism and support a model for aRPA functioning in chromosome maintenance functions in nonproliferating cells.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
