Product Name
SCN4B, siRNA
Full Product Name
SCN4B siRNA (Mouse)
Product Synonym Names
GM1471; Sodium channel subunit beta-4
Product Gene Name
SCN4B sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q7M729
Specificity
SCN4B siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SCN4B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of SCN4B sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
SCN4B sirna
siRNA to inhibit SCN4B expression using RNA interference
Applications Tested/Suitable for SCN4B sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for SCN4B. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001013408.1
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NCBI GenBank Nucleotide #
NM_001013390.2
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UniProt Primary Accession #
Q7M729
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UniProt Related Accession #
Q7M729[Other Products]
Molecular Weight
25,192 Da
NCBI Official Full Name
sodium channel subunit beta-4
NCBI Official Synonym Full Names
sodium channel, type IV, beta
NCBI Official Symbol
Scn4b [Similar Products]
NCBI Official Synonym Symbols
Gm1471
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NCBI Protein Information
sodium channel subunit beta-4
UniProt Protein Name
Sodium channel subunit beta-4
Protein Family
Sodium channel
UniProt Gene Name
Scn4b [Similar Products]
UniProt Synonym Gene Names
Gm1471 [Similar Products]
UniProt Entry Name
SCN4B_MOUSE
UniProt Comments for SCN4B
SCN4B: Modulates channel gating kinetics. Causes negative shifts in the voltage dependence of activation of certain alpha sodium channels, but does not affect the voltage dependence of inactivation. Defects in SCN4B are the cause of long QT syndrome type 10 (LQT10). Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress. They can present with a sentinel event of sudden cardiac death in infancy. Belongs to the sodium channel auxiliary subunit SCN4B (TC 8.A.17) family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Cellular Component: voltage-gated sodium channel complex; membrane; plasma membrane; integral to membrane; intrinsic to plasma membrane
Molecular Function: sodium channel regulator activity; voltage-gated sodium channel activity; voltage-gated ion channel activity; sodium channel activity
Biological Process: transport; sodium ion transport; ion transport; cardiac muscle contraction
Research Articles on SCN4B
1. NaVbeta4 plays a blocking role that indicates the relative importance of different residues in inducing open-channel block.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
