Nkx3.1, Monoclonal Antibody

Anti -Nkx3.1 (NK-2 Family Homeodomain Transcription Factor)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 8; NC_000008.10 (23536206..23540450, complement). Location: 8p21.2

Monoclonal

IgG1

6A141

Mouse

Recognizes human Nkx3.1 protein at ~34kD. Does not crossreact with mouse. Reactivity has been confirmed with human LN Cap-FGC prostate carcinoma cell lysates.

Affinity Purified
Purified by Protein A chromatography

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Small volumes of anti-NKX3-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

The Nkx3.1 protein is the product of a prostate-specific regulatory gene known as the NKX3.1 homeobox gene. NKX3.1 maps to chromosome band 8p21. Loss of heterozygosity at this particular site has been associated with tissue dedifferentiation and loss of androgen responsiveness in the progression of prostate cancer. Loss of specific regions of chromosome 8 occur in 60-80% of prostate tumors. Although a broad range of tumor suppressor genes, including NKX3.1, PTEN, Rb, p27, p16, and Myc, have been proposed as the genetic loci directly responsible for the chromosomal abnormalities characteristic of prostate cancer, none of these genes has consistently demonstrated mutations in a large population of prostate cancer specimens. Mouse studies of NKX3.1 have demonstrated that the gene is essential for normal prostate morphogenesis and function. Inactivation produces prostatic epithelial hyperplasia and dysplasia, closely modeling a preneoplastic condition. In vitro, overexpression of human and mouse NKX3.1 suppresses growth and tumorigenicity of prostate carcinoma cells. These findings suggest that NKX3.1 may be responsible for maintaining the normal prostate, and its loss may represent a predisposing event for prostate carcinogenesis.

Antibodies; Abs to Proteins

ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)

Suitable for use in Immunohistochemistry, Western Blot and ELISA.
Dilution: Immunohistochemistry (Frozen and paraffin): 10-20ug/ml
Western Blot: 1-3ug/ml
ELISA: 0.1-1ug/ml

26,350 Da[Similar Products]

NK3 homeobox 1

homeobox protein Nkx-3.1; OTTHUMP00000123534; NK homeobox, family 3, A; homeobox protein NK-3 homolog A; NK3 transcription factor homolog A; NK3 transcription factor related, locus 1

Homeobox protein Nkx-3.1

NKX3.1; NKX3A  [Similar Products]

NKX31_HUMAN

The homeodomain-containing transcription factor NKX3-1 is a putative prostate tumor suppressor that is expressed in a largely prostate-specific and androgen-regulated manner. Loss of NKX3-1 protein expression is a common finding in human prostate carcinomas and prostatic intraepithelial neoplasia.[supplied by OMIM]

NKX3-1: Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Play an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Act as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells. Interacts with serum response factor (SRF). Interacts with SPDEF. Interacts with WDR77. Interacts with TOPORS which polyubiquitinates NKX3-1 and induces its proteasomal degradation. By androgens and, in the LNCAP cell line, by estrogens. Androgenic control may be lost in prostate cancer cells during tumor progression from an androgen-dependent to an androgen- independent phase. Highly expressed in the prostate and, at a lower level, in the testis. Belongs to the NK-3 homeobox family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding; Tumor suppressor

Chromosomal Location of Human Ortholog: 8p21.2

Cellular Component: intracellular; nucleus

Molecular Function: protein binding; androgen receptor activity; estrogen receptor activity; protein self-association; sequence-specific DNA binding; histone deacetylase binding; estrogen receptor binding; caspase activator activity; protein kinase activator activity; transcription factor binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; salivary gland development; positive regulation of transcription, DNA-dependent; heart development; multicellular organismal development; negative regulation of insulin-like growth factor receptor signaling pathway; positive regulation of caspase activity; positive regulation of mitotic cell cycle; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; protein kinase B signaling cascade; negative regulation of mitotic cell cycle; negative regulation of epithelial cell proliferation; caspase activation; somitogenesis; pharyngeal system development; male gonad development; response to testosterone stimulus; positive regulation of phosphoinositide 3-kinase cascade; branching morphogenesis of a tube; positive regulation of cell division; positive regulation of protein kinase activity; androgen receptor signaling pathway; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; negative regulation of transcription, DNA-dependent; metanephros development

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