2-acylglycerol O-acyltransferase 1 (MOGAT1), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of MOGAT1. No significant cross-reactivity or interference between MOGAT1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MOGAT1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of MOGAT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7217698 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 2-acylglycerol O-acyltransferase 1 (MOGAT1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MOGAT1. The ELISA analytical biochemical technique of the MBS7217698 kit is based on MOGAT1 antibody-MOGAT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MOGAT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MOGAT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Intended Uses: This MOGAT1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse MOGAT1.

Principle of the Assay: MOGAT1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MOGAT1 antibody and an MOGAT1-HRP conjugate. The assay sample and buffer are incubated together with MOGAT1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MOGAT1 concentration since MOGAT1 from samples and MOGAT1-HRP conjugate compete for the anti-MOGAT1 antibody . Since the number of sites is limited, as more sites are occupied by MOGAT1 from the sample, fewer sites are left to bind MOGAT1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MOGAT1 concentration in each sample is interpolated from this standard curve.

Cardiovascular

38,812 Da

monoacylglycerol O-acyltransferase 1

2-acylglycerol O-acyltransferase 1; hDC2; diacylglycerol O-acyltransferase 2 like 1; acyl-CoA:monoacylglycerol acyltransferase 1; diacylglycerol O-acyltransferase candidate 2; diacylglycerol acyltransferase 2-like protein 1

2-acylglycerol O-acyltransferase 1

DC2; DGAT2L1; MGAT1; hDC2  [Similar Products]

MOGT1_HUMAN

Acyl-CoA:monoacylglycerol acyltransferase (MOGAT; EC 2.3.1.22) catalyzes the synthesis of diacylglycerols, the precursor of physiologically important lipids such as triacylglycerol and phospholipids (Yen et al., 2002 [PubMed 12077311]).[supplied by OMIM, Mar 2008]

MOGAT1: Catalyzes the formation of diacylglycerol from 2- monoacylglycerol and fatty acyl-CoA. Probably not involved in absorption of dietary fat in the small intestine. Belongs to the diacylglycerol acyltransferase family.

Protein type: EC 2.3.1.22; Endoplasmic reticulum; Membrane protein, multi-pass; Membrane protein, integral; Transferase

Chromosomal Location of Human Ortholog: 2q36.1

Cellular Component: endoplasmic reticulum membrane; integral to membrane

Molecular Function: 2-acylglycerol O-acyltransferase activity; diacylglycerol O-acyltransferase activity

Biological Process: diacylglycerol biosynthetic process; glycerol metabolic process; triacylglycerol biosynthetic process

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