Alcohol dehydrogenase class 4 mu/sigma chain (ADH7), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of ADH7 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7250850 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Alcohol dehydrogenase class 4 mu/sigma chain (ADH7) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADH7. The ELISA analytical biochemical technique of the MBS7250850 kit is based on ADH7 antibody-ADH7 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADH7 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADH7. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Cell Biology

42,467 Da

alcohol dehydrogenase 7 (class IV), mu or sigma polypeptide

alcohol dehydrogenase class 4 mu/sigma chain; retinol dehydrogenase; alcohol dehydrogenase-7; alcohol dehydrogenase VII; gastric alcohol dehydrogenase; class IV sigma-1 alcohol dehydrogenase; class IV sigmasigma alcohol dehydrogenase; alcohol dehydrogenase class IV mu/sigma chain

Alcohol dehydrogenase class 4 mu/sigma chain

ADH7_HUMAN

This gene encodes class IV alcohol dehydrogenase 7 mu or sigma subunit, which is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The enzyme encoded by this gene is inefficient in ethanol oxidation, but is the most active as a retinol dehydrogenase; thus it may participate in the synthesis of retinoic acid, a hormone important for cellular differentiation. The expression of this gene is much more abundant in stomach than liver, thus differing from the other known gene family members. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

ADH7: Could function in retinol oxidation for the synthesis of retinoic acid, a hormone important for cellular differentiation. Medium-chain (octanol) and aromatic (m-nitrobenzaldehyde) compounds are the best substrates. Ethanol is not a good substrate but at the high ethanol concentrations reached in the digestive tract, it plays a role in the ethanol oxidation and contributes to the first pass ethanol metabolism. Belongs to the zinc-containing alcohol dehydrogenase family. Class-IV subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Amino Acid Metabolism - tyrosine; Cofactor and Vitamin Metabolism - retinol; Oxidoreductase; EC 1.1.1.1; Xenobiotic Metabolism - metabolism by cytochrome P450; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Lipid Metabolism - fatty acid

Chromosomal Location of Human Ortholog: 4q23-q24

Cellular Component: extracellular region; cytosol

Molecular Function: receptor antagonist activity; zinc ion binding; aldehyde oxidase activity; alcohol dehydrogenase activity; alcohol dehydrogenase activity, zinc-dependent; ethanol binding; retinol binding; retinol dehydrogenase activity

Biological Process: response to ethanol; ethanol catabolic process; response to bacterium; retinol metabolic process; xenobiotic metabolic process; retinoic acid metabolic process; retinoid metabolic process; ethanol oxidation; fatty acid omega-oxidation

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