Lipocalin 2/NGAL, Polyclonal Antibody

LCN2; NGAL; lipocalin 2 (oncogene 24p3); HGNC: 6526; uterocalin; oncogenic lipocalin 24p3; neutrophil gelatinase-associated lipocalin; lipocalin 2; MSFI; OTTHUMP00000022240; migration-stimulating factor inhibitor; neutrophil gelatinase-associated lipocalin; NGAL antibody; lipocalin 2 (oncogene 24p3) antibody; HGNC: 6526 antibody; LCN2 antibody; uterocalin antibody; oncogenic lipocalin 24p3 antibody; neutrophil gelatinase-associated lipocalin antibody; lipocalin 2 antibody; MSFI antibody; OTTHUMP00000022240 antibody; migration-stimulating factor inhibitor antibody; neutrophil gelatinase-associated lipocalin antibody; oncogene 24p3 antibody; siderocalin antibody; Lipocalin 2; Lipocalin 2 / NGAL

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Goat

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul (lot specific)

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Manufactured in an ISO 9001:2008 Certified Laboratory.

Small volumes of anti-LCN2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Peptide ELISA: Antibody detection limit dilution 1: 16000.
Immunohistochemistry: In paraffin embedded Human Spleen shows staining of cytoplasm in mostly binucleated cells, and of nucleoli in some of the non-dividing cells. Recommended concentration, 2-3ug/ml.
Western Blot: Approx 22kDa band observed in Human Kidney lysates (calculated MW of 22.6kDa according to NP_005555.2). Recommended concentration: 0.01-0.03ug/ml.

(0. 03ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

(2.5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

22,457 Da

lipocalin 2

neutrophil gelatinase-associated lipocalin

Neutrophil gelatinase-associated lipocalin

HNL; NGAL; NGAL  [Similar Products]

NGAL_HUMAN

This gene encodes a protein that belongs to the lipocalin family. Members of this family transport small hydrophobic molecules such as lipids, steroid hormones and retinoids. The protein encoded by this gene is a neutrophil gelatinase-associated lipocalin and plays a role in innate immunity by limiting bacterial growth as a result of sequestering iron-containing siderophores. The presence of this protein in blood and urine is an early biomarker of acute kidney injury. This protein is thought to be be involved in multiple cellular processes, including maintenance of skin homeostasis, and suppression of invasiveness and metastasis. Mice lacking this gene are more susceptible to bacterial infection than wild type mice. [provided by RefSeq, Sep 2015]

LCN2: Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5- DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. Belongs to the calycin superfamily. Lipocalin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: cell; extracellular region; extracellular space

Molecular Function: iron ion binding; transporter activity

Biological Process: apoptosis; cellular iron ion homeostasis; innate immune response; ion transport; siderophore transport

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