Product Name
Glycine Receptor (GLRA1), Polyclonal Antibody
Full Product Name
Anti-Glycine Receptor
Product Gene Name
anti-GLRA1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 10; NC_005109.3 (40687069..40785100, complement). Location: 10q22
3D Structure
ModBase 3D Structure for P07727
Species Reactivity
Human, mouse, rat
Specificity
Specific for the ~48k alpha1- and alpha2-subunits of the glycine receptor in Western blots of rat spinal cord and brain stem and in cell extracts. Immunolabeling blocked by preadsorption of antibody with the peptide immunogen. Does not recognize other glycine receptor subunits.
Purity/Purification
Affinity Purified (Prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.)
Form/Format
200 ug antibody vial; lyophilized from 5 mM ammonium bicarbonate. The antibody should be reconstituted in 50 ul phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution the antibody s
Antigen
Peptide from the N-terminus region of the alpha1-subunit of rat glycine receptor.
Immunogen Information
Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH
Species Reactivity Note
The antibody has been directly tested for reactivity in Western blots in human, mouse and rat tissues.
Biological Significance
Glycine is an important inhibitory transmitter in the brainstem and spinal cord. Glycine receptors are members of the ligand-gated ion channel family (LGICs) that mediate rapid chemical neurotransmission (Schofield et al., 2003). The binding of glycine to its receptor produces a large increase in chloride conductance, which causes membrane hyperpolarization. Glycine receptors are anchored at inhibitory chemical synapses by a cytoplasmic protein, gephyrin (Fischer et al., 2000). The glycine receptor has been used to great advantage in the identification of the s for alcohol on the LGIC family of proteins (Beckstead et al., 2001; Mihic et al., 1997). These receptors have also been extremely useful in studies of synaptic clustering of receptors (Craig and Lichtman, 2001). The glycine receptor may also act in concert with an NMDAR subunit to form an excitatory receptor (Chatterton et al., 2002).
Preparation and Storage
For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.
Other Notes
Small volumes of anti-GLRA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-GLRA1 antibody
Affinity purified rabbit polyclonal antibody
Applications Tested/Suitable for anti-GLRA1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-GLRA1 antibody
Quality Control: Western blots performed on each lot.
WB: 1:1000
IHC (frozen sections; unpublished observations): 1:1000
Testing Data of anti-GLRA1 antibody
NCBI/Uniprot data below describe general gene information for GLRA1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_037265.1
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NCBI GenBank Nucleotide #
NM_013133.1
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UniProt Primary Accession #
P07727
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UniProt Secondary Accession #
Q546L6[Other Products]
UniProt Related Accession #
P07727[Other Products]
NCBI Official Full Name
glycine receptor subunit alpha-1
NCBI Official Synonym Full Names
glycine receptor, alpha 1
NCBI Official Symbol
Glra1 [Similar Products]
NCBI Official Synonym Symbols
GLYRA1
[Similar Products]
NCBI Protein Information
glycine receptor subunit alpha-1; glycine receptor 48 kDa subunit; glycine receptor, alpha 1 subunit; glycine receptor strychnine-binding subunit
UniProt Protein Name
Glycine receptor subunit alpha-1
UniProt Synonym Protein Names
Glycine receptor 48 kDa subunit; Glycine receptor strychnine-binding subunit
Protein Family
Glycine receptor
UniProt Gene Name
Glra1 [Similar Products]
UniProt Synonym Gene Names
Glyr [Similar Products]
UniProt Entry Name
GLRA1_RAT
NCBI Summary for GLRA1
glycine receptor strychnine binding subunit; generates glycine gated strychnine sensitive chloride current in the central nervous system [RGD, Feb 2006]
UniProt Comments for GLRA1
Function: The glycine receptor is a neurotransmitter-gated ion channel. Binding of glycine to its receptor increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing).
Subunit structure: Pentamer composed of three alpha and two beta subunits. Homopentamers of alpha subunits also form functional receptors.
Subcellular location: Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
Miscellaneous: The alpha subunit binds strychnine.
Sequence similarities: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Glycine receptor (TC 1.A.9.3) subfamily. GLRA1 sub-subfamily. [View classification]
Product References and Citations for anti-GLRA1 antibody
• Beckstead MJ, Phelan R, Mihic SJ (2001) Antagonism of inhalant and volatile anesthetic enhancement of glycine receptor function. J Biol Chem 276:24959-24964.
• Chatterton JE, Awobuluyi M, Premkumar LS, Takahashi H, Talantova M, Shin Y, Cui JK, Tu SC, Kevin ASK, Nakanishi N, Tong G, Lipton SA, Zhang DX (2002) Excitatory glycine receptors containing the NR3 family of NMDA receptor subunits. Nature (London) 415:793-798.
• Craig AM, Lichtman JW (2001) Getting a bead on receptor movements. Nat Neurosci 4:219-220.
• Fischer F, Kneussel M, Tintrup H, Haverkamp S, Rauen T, Betz H, Wässle H (2000) Reduced synaptic clustering of GABA and glycine receptors in the retina of the gephyrin null mutant mouse. J Comp Neurol 427:634-648.
• Mihic SJ, Ye Q, Wick MJ, Koltchine VV, Krasowski MD, Finn SE, Mascia MP, Valenzuela CF, Hanson KK, Greenblatt EP, Harris RA, Harrison NL (1997) Sites of alcohol and volatile anaesthetic action on GABAA and glycine receptors. Nature (London) 389:385-389.
Research Articles on GLRA1
1. overexpressing the Glra1 subunite in rat neurons caused reduced potentition and enhanced inhibition by zinc.
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