Full Product Name
DNM2 siRNA (Mouse)
Product Synonym Names
DYN2; Dynamin-2; Dynamin UDNM
Product Gene Name
DNM2 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P39054
Specificity
DNM2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse DNM2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of DNM2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
DNM2 sirna
siRNA to inhibit DNM2 expression using RNA interference
Applications Tested/Suitable for DNM2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for DNM2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001240822.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001253893.1
[Other Products]
UniProt Primary Accession #
P39054
[Other Products]
UniProt Secondary Accession #
Q9DBE1[Other Products]
UniProt Related Accession #
P39054[Other Products]
Molecular Weight
97,733 Da
NCBI Official Full Name
dynamin-2 isoform 1
NCBI Official Synonym Full Names
dynamin 2
NCBI Official Symbol
Dnm2 [Similar Products]
NCBI Official Synonym Symbols
Dyn2; Udnm; b2b2159Clo
[Similar Products]
NCBI Protein Information
dynamin-2
UniProt Protein Name
Dynamin-2
UniProt Synonym Protein Names
Dynamin UDNM
UniProt Gene Name
Dnm2 [Similar Products]
UniProt Synonym Gene Names
Dyn2 [Similar Products]
UniProt Entry Name
DYN2_MOUSE
UniProt Comments for DNM2
DYN2: a cytoplasmic microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes. May mediate the conventional clathrin-mediated uptake of surface receptors. Expressed in the postsynaptic density of neuronal cells. Two alternatively spliced isoforms have been described.
Protein type: EC 3.6.5.5; Hydrolase; Microtubule-binding; Vesicle; Motility/polarity/chemotaxis
Cellular Component: Golgi apparatus; centrosome; microtubule; focal adhesion; protein complex; photoreceptor inner segment; coated pit; trans-Golgi network; clathrin-coated endocytic vesicle; cytosol; Golgi membrane; postsynaptic membrane; cytoskeleton; growth cone; membrane; perinuclear region of cytoplasm; lamellipodium; cytoplasm; plasma membrane; synapse; nucleus; cell junction; endosome; phagocytic cup
Molecular Function: GTPase activity; protein binding; GTP binding; hydrolase activity; protein complex binding; WW domain binding; nucleotide binding; nitric-oxide synthase binding; protein kinase binding; SH3 domain binding; D2 dopamine receptor binding
Biological Process: receptor-mediated endocytosis; Golgi to plasma membrane transport; regulation of Rac protein signal transduction; positive regulation of nitric oxide biosynthetic process; G-protein coupled receptor internalization; positive regulation of phagocytosis; receptor internalization; transferrin transport; endocytosis; positive regulation of endocytosis; regulation of axon extension; negative regulation of transforming growth factor beta receptor signaling pathway; neurite morphogenesis
Research Articles on DNM2
1. Dynamin 2 has a role in endocytosis and in sustaining T-cell receptor signaling and driving metabolic reprogramming in T lymphocytes
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
