Product Name
Ghrelin (GHRL), Polyclonal Antibody
Full Product Name
Ghrelin Antibody: Dylight 350
Product Synonym Names
Rabbit Anti-Human Ghrelin Polyclonal; Appetite-regulating hormone Antibody; ghrl Antibody; Ghrelin 28 Antibody; Protein M46 Antibody; GHRL_HUMAN Antibody; GHRL Antibody; Ghrelin Antibody; Growth hormone-releasing peptide Antibody; Motilin-related peptide Antibody; Appetite regulating hormone Antibody; Growth hormone releasing peptide Antibody; Growth hormone secretagogue Antibody; Motilin related peptide Antibody; Ghrelin/obestatin prepropeptide Antibody; Obestatin Antibody; MTLRP Antibody; Ghrelin 27 Antibody; M46 protein Antibody; Ghrelin
Product Gene Name
anti-GHRL antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9UBU3
Species Reactivity
Human, Rat
Specificity
Detects ~13 kDa.
Purity/Purification
Peptide Affinity Purified
Form/Format
PBS, 50% Glycerol, 0.09% Sodium Azide
Concentration
1 mg/ml (lot specific)
Immunogen
Synthetic peptide from the mid-protein of Human Ghrelin
Cellular Localization
Secreted
Tissue Specificity
Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy.
Certificate of Analysis
A 1:1000 dilution was sufficient for detection of Ghrelin in 15 ug of rat Pancreas cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Product Notes
Conjugated primary antibodies can be good options or alternatives to secondary antibody's for detecting a target protein. The lab manufactures a conjugated antibody from a primary parent antibody by attaching (conjugating) an available tag such as a fluorophore or protein, allowing detection of the target protein without using a secondary antibody. Conjugated primary antibodies may potentially be used to replace primary/secondary antibody pairs in various applications, such as Enzyme-Linked Immunosorbent Assay (ELISA), Flow Cytometry, Fluorescence-activated cell sorting, Förster Resonance Energy Transfer (FRET). Immunocytochemistry, Immunofluorescence, Immunohistochemistry, or Western Blot. The suitability of applications for conjugates are user determined and we cannot guarantee that any antibody-conjugate combination has been tested for use in a particular application.
Please note: Conjugate tags may potentially bind in the antibody paratope, which can limit binding of the antibody to the antigen. Binding in the antibody paratope may affect the ability of the antibody to bind to the corresponding antigen (protein target) in various species and applications. There is no way to confirm or guarantee the location of the antibody tag as we cannot control the binding of the conjugate to the antibody. Researchers are responsible for evaluating antibody conjugates for the suitability of their applications.
Preparation and Storage
Store at-20 degree C.
Other Notes
Small volumes of anti-GHRL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-GHRL antibody
Cardiovascular System; Blood; Cardiovascular System; Atherosclerosis; Diabetes Associated; Neuroscience; Cardiovascular System; Angiogenesis; Cardiovascular Growth Factors
Applications Tested/Suitable for anti-GHRL antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes for anti-GHRL antibody
WB: 1:1000
ICC/IF: 1:100
IHC: 1:50
Immunocytochemistry (ICC) of anti-GHRL antibody
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Ghrelin Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Ghrelin Polyclonal Antibody. Tissue: Stomach. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-Ghrelin Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Pancreas cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Ghrelin Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Ghrelin Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Ghrelin Antibody. (D) Composite.
NCBI/Uniprot data below describe general gene information for GHRL. It may not necessarily be applicable to this product.
NCBI Accession #
Q9UBU3.1
[Other Products]
UniProt Primary Accession #
Q9UBU3
[Other Products]
UniProt Related Accession #
Q9UBU3[Other Products]
NCBI Official Full Name
Appetite-regulating hormone
NCBI Official Synonym Full Names
ghrelin and obestatin prepropeptide
NCBI Official Symbol
GHRL [Similar Products]
NCBI Official Synonym Symbols
MTLRP
[Similar Products]
NCBI Protein Information
appetite-regulating hormone
UniProt Protein Name
Appetite-regulating hormone
UniProt Synonym Protein Names
Growth hormone secretagogue; Growth hormone-releasing peptide; Motilin-related peptide; Protein M46Cleaved into the following 3 chains:Ghrelin-27; Ghrelin-28; Ghrelin; Obestatin
UniProt Gene Name
GHRL [Similar Products]
UniProt Synonym Gene Names
MTLRP; Ghrelin [Similar Products]
UniProt Entry Name
GHRL_HUMAN
NCBI Summary for GHRL
This gene encodes the ghrelin-obestatin preproprotein that is cleaved to yield two peptides, ghrelin and obestatin. Ghrelin is a powerful appetite stimulant and plays an important role in energy homeostasis. Its secretion is initiated when the stomach is empty, whereupon it binds to the growth hormone secretagogue receptor in the hypothalamus which results in the secretion of growth hormone (somatotropin). Ghrelin is thought to regulate multiple activities, including hunger, reward perception via the mesolimbic pathway, gastric acid secretion, gastrointestinal motility, and pancreatic glucose-stimulated insulin secretion. It was initially proposed that obestatin plays an opposing role to ghrelin by promoting satiety and thus decreasing food intake, but this action is still debated. Recent reports suggest multiple metabolic roles for obestatin, including regulating adipocyte function and glucose metabolism. Alternative splicing results in multiple transcript variants. In addition, antisense transcripts for this gene have been identified and may potentially regulate ghrelin-obestatin preproprotein expression. [provided by RefSeq, Nov 2014]
UniProt Comments for GHRL
ghrelin: a hormone that binds to the growth hormone secretagogue receptor type 1 (GHSR). Secreted by the stomach
Protein type: Secreted; Cell development/differentiation; Cell cycle regulation; Secreted, signal peptide; Apoptosis
Chromosomal Location of Human Ortholog: 3p26-p25
Cellular Component: extracellular space; endoplasmic reticulum lumen; axon; extracellular region
Molecular Function: growth hormone-releasing hormone activity; G-protein-coupled receptor binding; ghrelin receptor binding; protein tyrosine kinase activator activity
Biological Process: cortisol secretion; negative regulation of circadian sleep/wake cycle, REM sleep; positive regulation of cortisol secretion; hormone-mediated signaling; activation of MAPK activity; response to hormone stimulus; negative regulation of interleukin-6 biosynthetic process; positive regulation of multicellular organism growth; decidualization; positive regulation of adrenocorticotropic hormone secretion; negative regulation of insulin secretion; growth hormone secretion; elevation of cytosolic calcium ion concentration; negative regulation of locomotion; positive regulation of appetite; dendrite development; negative regulation of tumor necrosis factor biosynthetic process; positive regulation of circadian sleep/wake cycle, non-REM sleep; regulation of response to food; gastric acid secretion; positive regulation of insulin secretion; glucose metabolic process; ***** feeding behavior; negative regulation of interleukin-1 beta production; regulation of cell proliferation; positive regulation of growth hormone secretion; positive regulation of synaptogenesis; G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; cellular protein metabolic process; response to estrogen stimulus; negative regulation of inflammatory response; cartilage development; actin polymerization and/or depolymerization; negative regulation of endothelial cell proliferation; regulation of excitatory postsynaptic membrane potential; negative regulation of apoptosis
Disease: Obesity
Research Articles on GHRL
1. feedback between the level of ghrelin and gastrin in the blood of patients with chronic gastritis and type 2 diabetes mellitus; with increasing levels of gastrin in the blood, the level of ghrelin in the blood decreases and vice versa
Precautions
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