Full Product Name
PLCG1 (Phospho-Tyr977) Antibody
Product Synonym Names
1 phosphatidyl D myo inositol 4 5 bisphosphate; 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1; 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase gamma-1; Inositoltrisphosphohydrolase; Monophosphatidylinositol phosphodiesterase; NCKAP3; Phosphatidylinositol phospholipase C; Phosphoinositidase C; Phosphoinositide phospholipase C; Phosphoinositide phospholipase C-gamma-1; Phospholipase C 148; Phospholipase C gamma 1; Phospholipase C-gamma-1; Phospholipase C-II; PLC gamma 1; PLC II; PLC-148; PLC-gamma-1; PLC-II; PLC1; PLC148; Plcg1; PLCG1_HUMAN; PLCgamma1;
Product Gene Name
anti-PLCG1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P19174
Species Reactivity
Human, Mouse, Rat
Specificity
Phospho-PLCG1 (Phospho-Tyr977) Antibody detects endogenous levels of PLCG1 only when phosphorylated at Tyr977
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Form/Format
In phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0mg/mL (lot specific)
Immunogen
A synthesized peptide derived from human PLCG1 around the phosphorylation site of Tyr977
Preparation and Storage
Store at -20 degree C.
Other Notes
Small volumes of anti-PLCG1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-PLCG1 antibody
Phospho-specific Ab
Applications Tested/Suitable for anti-PLCG1 antibody
Western Blot (WB)
Application Notes for anti-PLCG1 antibody
WB: 1:500-1:2000
Western Blot (WB) of anti-PLCG1 antibody
Western blot analysis of Phospho-PLCG1 (Tyr977) in lysates of COS7 EGF 200ng/ml 30', using Phospho-PLCG1 (Tyr977) Antibody(13108).
NCBI/Uniprot data below describe general gene information for PLCG1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_002651.2
[Other Products]
NCBI GenBank Nucleotide #
NM_002660.2
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UniProt Primary Accession #
P19174
[Other Products]
UniProt Secondary Accession #
Q2V575; B7ZLY7; B9EGH4; E1P5W4[Other Products]
UniProt Related Accession #
P19174[Other Products]
NCBI Official Full Name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 isoform a
NCBI Official Synonym Full Names
phospholipase C gamma 1
NCBI Official Symbol
PLCG1 [Similar Products]
NCBI Official Synonym Symbols
PLC1; NCKAP3; PLC-II; PLC148; PLCgamma1
[Similar Products]
NCBI Protein Information
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1
UniProt Protein Name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1
UniProt Synonym Protein Names
PLC-148; Phosphoinositide phospholipase C-gamma-1; Phospholipase C-II; PLC-II; Phospholipase C-gamma-1; PLC-gamma-1
Protein Family
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
UniProt Gene Name
PLCG1 [Similar Products]
UniProt Synonym Gene Names
PLC1; PLC-II; PLC-gamma-1 [Similar Products]
NCBI Summary for PLCG1
The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for PLCG1
Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, FGFR1, FGFR2, FGFR3 and FGFR4. Plays a role in actin reorganization and cell migration.
Research Articles on PLCG1
1. Syk-induced signals in bone marrow stromal cell lines are mediated by phospholipase C gamma1 (PLCgamma1) in osteogenesis and PLCgamma2 in adipogenesis.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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