Full Product Name
PKLR siRNA (Rat)
Product Synonym Names
Pyruvate kinase PKLR; L-PK; Pyruvate kinase isozymes L/R
Product Gene Name
PKLR sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P12928
Specificity
PKLR siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat PKLR gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of PKLR sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
PKLR sirna
siRNA to inhibit PKLR expression using RNA interference
Applications Tested/Suitable for PKLR sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for PKLR. It may not necessarily be applicable to this product.
NCBI Accession #
NP_036756.3
[Other Products]
NCBI GenBank Nucleotide #
NM_012624.3
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UniProt Primary Accession #
P12928
[Other Products]
UniProt Secondary Accession #
P04763; Q64618[Other Products]
UniProt Related Accession #
P12928[Other Products]
Molecular Weight
58,794 Da
NCBI Official Full Name
pyruvate kinase PKLR
NCBI Official Synonym Full Names
pyruvate kinase, liver and RBC
NCBI Official Symbol
Pklr [Similar Products]
NCBI Official Synonym Symbols
PK1; PKL; Pklg
[Similar Products]
NCBI Protein Information
pyruvate kinase PKLR
UniProt Protein Name
Pyruvate kinase PKLR
UniProt Synonym Protein Names
L-PK; Pyruvate kinase isozymes L/R
Protein Family
Pyruvate kinase
UniProt Gene Name
Pklr [Similar Products]
UniProt Entry Name
KPYR_RAT
NCBI Summary for PKLR
L-isozyme of pyruvate kinase; catalyzes the conversion of ATP and pyruvate to ADP and phosphoenolpyruvate in glycolysis [RGD, Feb 2006]
UniProt Comments for PKLR
PKLR: one of three rate-limiting enzymes in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule ATP and a pyruvate molecule, which is a central metabolic intermediate that can be used as a building block or oxidized further. There are several mammalian isozymes of pyruvate kinase encoded by different genes. The L type predominates in liver, and the M type in muscle and brain. Two alternatively spliced human isoforms have been described: the R- and L-types.
Protein type: Carbohydrate Metabolism - pyruvate; Kinase, other; Nucleotide Metabolism - purine; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 2.7.1.40
Cellular Component: cytosol
Molecular Function: potassium ion binding; magnesium ion binding; pyruvate kinase activity; ATP binding
Biological Process: pyruvate biosynthetic process; response to metal ion; response to cAMP; glycolysis; response to lithium ion; response to other organism; response to organic cyclic substance; response to ATP; cellular response to insulin stimulus; response to heat; ATP biosynthetic process; carbohydrate metabolic process; response to glucose stimulus; response to hypoxia; phosphorylation; response to nutrient
Research Articles on PKLR
1. Studied the effect of the point mutations around this phosphorylation site on the catalytic properties of L-pyruvate kinase.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
