Product Name
PKLR, cDNA Clone
Full Product Name
PKLR cDNA Clone
Product Gene Name
PKLR cdna clone
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
atgtcgatcc aggagaacat atcatccctg cagcttcggt catgggtctc taagtcccaa agagacttag caaagtccat cctgattggg gctccaggag ggccagcggg gtatctgcgg cgggccagtg tggcccaact gacccaggag ctgggcactg ccttcttcca gcagcagcag ctgccagctg ctatggcaga caccttcctg gaacacctct gcctactgga cattgactcc gagcccgtgg ctgctcgcag taccagcatc attgccacca tcgggccagc atctcgctcc gtggagcgcc tcaaggagat gatcaaggcc gggatgaaca ttgcgcgact caacttctcc cacggctccc acgagtacca tgctgagtcc atcgccaacg tccgggaggc ggtggagagc tttgcaggtt ccccactcag ctaccggccc gtggccatcg ccctggacac caagggaccg gagatccgca ctgggatcct gcaggggggt ccagagtcgg aagtggagct ggtgaagggc tcccaggtgc tggtgactgt ggaccccgcg ttccggacgc gggggaacgc gaacaccgtg tgggtggact accccaatat tgtccgggtc gtgccggtgg ggggccgcat ctacattgac gacgggctca tctccctagt ggtccagaaa atcggcccag agggactggt gacccaagtg gagaacggcg gcgtcctggg cagccggaag ggcgtgaact tgccaggggc ccaggtggac ttgcccgggc tgtccgagca ggacgtccga gacctgcgct tcggggtgga gcatggggtg gacatcgtct ttgcctcctt tgtgcggaaa gccagcgacg tggctgccgt cagggctgct ctgggtccgg aaggacacgg catcaagatc atcagcaaaa ttgagaacca cgaaggcgtg aagaggtttg atgaaatcct ggaggtgagc gacggcatca tggtggcacg gggggaccta ggcatcgaga tcccagcaga gaaggttttc ctggctcaga agatgatgat tgggcgctgc aacttggcgg gcaagcctgt tgtctgtgcc acacagatgc tggagagcat gattaccaag ccccggccaa cgagggcaga gacaagcgat gtcgccaatg ctgtgctgga tggggctgac tgcatcatgc tgtcagggga gactgccaag ggcaacttcc ctgtggaagc ggtgaagatg cagcatgcga ttgcccggga ggcagaggcc gcagtgtacc accggcagct gtttgaggag ctacgtcggg cagcgccact aagccgtgat cccactgagg tcaccgccat tggtgctgtg gaggctgcct tcaagtgctg tgctgctgcc atcattgtgc tgaccacaac tggccgctca gcccagcttc tgtctcggta ccgacctcgg gcagcagtca ttgctgtcac ccgctctgcc caggctgccc gccaggtcca cttatgccga ggagtcttcc ccttgcttta ccgtgaacct ccagaagcca tctgggcaga tgatgtagat cgccgggtgc aatttggcat tgaaagtgga aagctccgtg gcttcctccg tgttggagac ctggtgattg tggtgacagg ctggcgacct ggctccggct acaccaacat catgagggtg ctaagcatat cctga
Clone Sequence Report
Provided with product shipment
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of PKLR cdna clone vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
NCBI/Uniprot data below describe general gene information for PKLR. It may not necessarily be applicable to this product.
NCBI Accession #
BC025737
[Other Products]
UniProt Secondary Accession #
O75758; P11973[Other Products]
UniProt Related Accession #
P30613[Other Products]
Molecular Weight
58,494 Da
NCBI Official Full Name
Homo sapiens pyruvate kinase, liver and RBC, mRNA
NCBI Official Synonym Full Names
pyruvate kinase, liver and RBC
NCBI Official Symbol
PKLR [Similar Products]
NCBI Official Synonym Symbols
PK1; PKL; PKR; RPK; PKRL
[Similar Products]
NCBI Protein Information
pyruvate kinase PKLR
UniProt Protein Name
Pyruvate kinase PKLR
UniProt Synonym Protein Names
Pyruvate kinase 1; Pyruvate kinase isozymes L/R; R-type/L-type pyruvate kinase; Red cell/liver pyruvate kinase
Protein Family
Pyruvate kinase
UniProt Gene Name
PKLR [Similar Products]
UniProt Synonym Gene Names
PK1; PKL [Similar Products]
UniProt Entry Name
KPYR_HUMAN
NCBI Summary for PKLR
The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for PKLR
PKLR: one of three rate-limiting enzymes in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule ATP and a pyruvate molecule, which is a central metabolic intermediate that can be used as a building block or oxidized further. There are several mammalian isozymes of pyruvate kinase encoded by different genes. The L type predominates in liver, and the M type in muscle and brain. Two alternatively spliced human isoforms have been described: the R- and L-types.
Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 2.7.1.40; Nucleotide Metabolism - purine; Kinase, other; Carbohydrate Metabolism - pyruvate
Chromosomal Location of Human Ortholog: 1q21
Cellular Component: cytosol
Molecular Function: pyruvate kinase activity
Disease: Adenosine Triphosphate, Elevated, Of Erythrocytes; Pyruvate Kinase Deficiency Of Red Cells
Research Articles on PKLR
1. Two Novel Missense Mutations and a 5bp Deletion in the Erythroid-Specific Promoter of the PKLR Gene in Two Unrelated Patients With Pyruvate Kinase Deficient Transfusion-Dependent Chronic Nonspherocytic Hemolytic Anemia.
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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