Choline acetyltransterase (ChAT), ELISA Kit

CMS1A; CMS1A2; acetyl CoA:choline O-acetyltransferase

For Research Use Only. Not for use in diagnostic procedures.

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Small volumes of ChAT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2800178 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Choline acetyltransterase (ChAT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ChAT. The ELISA analytical biochemical technique of the MBS2800178 kit is based on ChAT antibody-ChAT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ChAT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ChAT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

This assay employs a two-site sandwich ELISA to quantitate CHAT in samples. An antibody specific for CHAT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CHAT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHAT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHAT bound in the initial step. The color development is stopped and the intensity of the color is measured.

70,394 Da

choline O-acetyltransferase

choline O-acetyltransferase

Choline O-acetyltransferase

CHOACTase; ChAT; Choline acetylase  [Similar Products]

This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]

Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

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