Alpha-N-acetylglucosaminidase, ELISA Kit

NAGLU (N-Acetyl Alpha-D-Glucosaminidase)/NAG/UFHSD1/MPS3B/MPS-IIIB/N-acetyl-alpha-glucosaminidase/NAGalpha-N-acetylglucosaminidase/UFHSD

For Research Use Only. Not for use in diagnostic procedures.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of NAGLU elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS760425 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Alpha-N-acetylglucosaminidase (NAGLU) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NAGLU. The ELISA analytical biochemical technique of the MBS760425 kit is based on NAGLU antibody-NAGLU antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NAGLU antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NAGLU. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- NAGLU antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- NAGLU antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the NAGLU amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of NAGLU can be calculated.

82,266 Da

N-acetylglucosaminidase, alpha

alpha-N-acetylglucosaminidase

Alpha-N-acetylglucosaminidase

UFHSD1; NAG  [Similar Products]

ANAG_HUMAN

This gene encodes an enzyme that degrades heparan sulfate by hydrolysis of terminal N-acetyl-D-glucosamine residues in N-acetyl-alpha-D-glucosaminides. Defects in this gene are the cause of mucopolysaccharidosis type IIIB (MPS-IIIB), also known as Sanfilippo syndrome B. This disease is characterized by the lysosomal accumulation and urinary excretion of heparan sulfate. [provided by RefSeq, Jul 2008]

NAGLU: Involved in the degradation of heparan sulfate. Defects in NAGLU are the cause of mucopolysaccharidosis type 3B (MPS3B); also known as Sanfilippo syndrome B. MPS3B is a form of mucopolysaccharidosis type 3, an autosomal recessive lysosomal storage disease due to impaired degradation of heparan sulfate. MPS3 is characterized by severe central nervous system degeneration, but only mild somatic disease. Onset of clinical features usually occurs between 2 and 6 years; severe neurologic degeneration occurs in most patients between 6 and 10 years of age, and death occurs typically during the second or third decade of life.

Protein type: Hydrolase; Glycan Metabolism - glycosaminoglycan degradation; EC 3.2.1.50

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: lysosomal lumen; lysosome

Molecular Function: alpha-N-acetylglucosaminidase activity

Biological Process: carbohydrate metabolic process; cerebellar Purkinje cell layer development; glycosaminoglycan catabolic process; glycosaminoglycan metabolic process; inner ear receptor cell development; locomotor rhythm; lysosome organization and biogenesis; middle ear morphogenesis; nervous system development; retinal rod cell development

Disease: Charcot-marie-tooth Disease, Axonal, Type 2v; Mucopolysaccharidosis, Type Iiib

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