Full Product Name
SS18 siRNA (Mouse)
Product Synonym Names
SSXT; SYT; Protein SSXT; Protein SYT; Synovial sarcoma-associated Ss18-alpha
Product Gene Name
SS18 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q62280
Specificity
SS18 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SS18 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of SS18 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
SS18 sirna
siRNA to inhibit SS18 expression using RNA interference
Applications Tested/Suitable for SS18 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for SS18. It may not necessarily be applicable to this product.
NCBI Accession #
NP_033306.2
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NCBI GenBank Nucleotide #
NM_009280.2
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UniProt Primary Accession #
Q62280
[Other Products]
UniProt Secondary Accession #
Q3TM96[Other Products]
UniProt Related Accession #
Q62280[Other Products]
Molecular Weight
45,864 Da
NCBI Official Full Name
protein SSXT isoform 1
NCBI Official Synonym Full Names
synovial sarcoma translocation, Chromosome 18
NCBI Official Symbol
Ss18 [Similar Products]
NCBI Official Synonym Symbols
Syt; Ssxt; D130059H17
[Similar Products]
NCBI Protein Information
protein SSXT
UniProt Protein Name
Protein SSXT
UniProt Synonym Protein Names
Protein SYT; Synovial sarcoma-associated Ss18-alpha
Protein Family
SS18-like protein
UniProt Gene Name
Ss18 [Similar Products]
UniProt Synonym Gene Names
Ssxt; Syt [Similar Products]
UniProt Entry Name
SSXT_MOUSE
UniProt Comments for SS18
SS18: Appears to function synergistically with RBM14 as a transcriptional coactivator. Isoform 1 and isoform 2 function in nuclear receptor coactivation. Isoform 1 and isoform 2 function in general transcriptional coactivation. A chromosomal aberration involving SS18 may be a cause of synovial sarcoma. Translocation t(X;18)(p11.2;q11.2). The translocation is specifically found in more than 80% of synovial sarcoma. The fusion products SSXT-SSX1 or SSXT-SSX2 are probably responsible for transforming activity. Heterogeneity in the position of the breakpoint can occur (low frequency). Belongs to the SS18 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Nuclear receptor co-regulator; Oncoprotein; Transcription, coactivator/corepressor
Cellular Component: cytoplasmic microtubule; nucleus
Molecular Function: ligand-dependent nuclear receptor transcription coactivator activity
Biological Process: response to drug; regulation of transcription, DNA-dependent; transcription, DNA-dependent; ephrin receptor signaling pathway; cell morphogenesis; positive regulation of transcription from RNA polymerase II promoter; cytoskeleton organization and biogenesis; microtubule cytoskeleton organization and biogenesis
Research Articles on SS18
1. Syt may contribute to the signaling pathway important for various cellular functions in vivo and in vitro, and we propose that Syt-deficient MEFs would be a powerful means to understand the
biological roles of SYT in vitro.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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