EphB1 / EPHT2, Recombinant Protein

9330129L11, AW488255, C130099E04Rik, ENSMUSG00000074119, Cek6, Elk, Elkh, Hek6, Net

For Research Use Only. Not for use in diagnostic procedures.

Baculovirus-Insect Cells

> 95 % as determined by SDS-PAGE

Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly

Samples are stable for up to twelve months from date of receipt at -70 degree C

Manufactured in an ISO 9001:2015 Certified Laboratory.

Small volumes of EphB1 / EPHT2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Background: Ephrin type -B receptor 1, also known as EphB1, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.

Description: A DNA sequence encoding the cytoplasmic domain (Met 591-Ala 984) of mouse EPHB1 (Q8CBF3-1) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

The secreted recombinant mouse EPHB1/GST chimera consists of 631 amino acids and has a calculated molecular mass of 72.4 kDa. The recombinant protein migrates as an approximately 60 kDa band in SDS-PAGE under reducing conditions.

105,294 Da

Eph receptor B1

ephrin type-B receptor 1

Ephrin type-B receptor 1

EphB1: a receptor tyrosine kinase of the Eph family. Receptor for members of the ephrin-B family: ephrin-B1, -B2 and -B3. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. The ligand-activated form of EphB1 interacts with GRB2, GRB10 and NCK through their respective SH2 domains. Four alternatively spliced isoforms are known.

Protein type: EC 2.7.10.1; Eph family; Kinase, protein; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group

Chromosomal Location of Human Ortholog: 9|9 F1

Cellular Component: axon; cytoplasm; early endosome membrane; filopodium tip; integral to plasma membrane; lipid raft; membrane

Molecular Function: axon guidance receptor activity; protein binding; protein complex binding; protein-tyrosine kinase activity; transmembrane-ephrin receptor activity

Biological Process: angiogenesis; axon guidance; camera-type eye morphogenesis; cell-substrate adhesion; central nervous system projection neuron axonogenesis; cranial nerve development; detection of temperature stimulus involved in sensory perception of pain; ephrin receptor signaling pathway; establishment of cell polarity; formation of immunological synapse; neurogenesis; optic nerve morphogenesis; positive regulation of synaptogenesis; protein amino acid autophosphorylation; regulation of JNK cascade; retinal ganglion cell axon guidance

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.