Product Name
ARFGEF2, siRNA
Full Product Name
ARFGEF2 siRNA (Rat)
Product Synonym Names
ARFGEP2; BIG2; Brefeldin A-inhibited guanine nucleotide-exchange protein 2; Brefeldin A-inhibited GEP 2; ADP-ribosylation factor guanine nucleotide-exchange factor 2
Product Gene Name
ARFGEF2 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q7TSU1
Specificity
ARFGEF2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat ARFGEF2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ARFGEF2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ARFGEF2 sirna
siRNA to inhibit ARFGEF2 expression using RNA interference
Applications Tested/Suitable for ARFGEF2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ARFGEF2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_851597.2
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NCBI GenBank Nucleotide #
NM_181083.2
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UniProt Primary Accession #
Q7TSU1
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UniProt Related Accession #
Q7TSU1[Other Products]
Molecular Weight
201,974 Da
NCBI Official Full Name
brefeldin A-inhibited guanine nucleotide-exchange protein 2
NCBI Official Synonym Full Names
ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited)
NCBI Official Symbol
Arfgef2 [Similar Products]
NCBI Official Synonym Symbols
Big2
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NCBI Protein Information
brefeldin A-inhibited guanine nucleotide-exchange protein 2
UniProt Protein Name
Brefeldin A-inhibited guanine nucleotide-exchange protein 2
UniProt Synonym Protein Names
ADP-ribosylation factor guanine nucleotide-exchange factor 2
UniProt Gene Name
Arfgef2 [Similar Products]
UniProt Synonym Gene Names
Arfgep2; Big2; Brefeldin A-inhibited GEP 2 [Similar Products]
UniProt Entry Name
BIG2_RAT
UniProt Comments for ARFGEF2
ARFGEF2: Promotes guanine-nucleotide exchange on ARF1 and ARF3 and to a lower extend on ARF5 and ARF6. Promotes the activation of ARF1/ARF5/ARF6 through replacement of GDP with GTP. Involved in the regulation of Golgi vesicular transport. Required for the integrity of the endosomal compartment. Involved in trafficking from the trans-Golgi network (TGN) to endosomes and is required for membrane association of the AP-1 complex and GGA1. Seems to be involved in recycling of the transferrin receptor from recycling endosomes to the plasma membrane. Probably is involved in the exit of GABA(A) receptors from the endoplasmic reticulum. Involved in constitutive release of tumor necrosis factor receptor 1 via exosome-like vesicles; the function seems to involve PKA and specifically PRKAR2B. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways. Defects in ARFGEF2 are the cause of autosomal recessive periventricular nodular heterotopia type 2 (PVNH2); also known as periventricular heterotopia with microcephaly autosomal recessive. PVNH is a developmental disorder characterized by the presence of periventricular nodules of cerebral gray matter, resulting from a failure of neurons to migrate normally from the lateral ventricular proliferative zone, where they are formed, to the cerebral cortex. PVNH2 is an autosomal recessive form characterized by microcephaly (small brain), severe developmental delay and recurrent infections. No anomalies extrinsic to the central nervous system, such as dysmorphic features or grossly abnormal endocrine or other conditions, are associated with PVNH2.
Protein type: GEFs, ARF; GEFs
Cellular Component: asymmetric synapse; Golgi membrane; recycling endosome; membrane; perinuclear region of cytoplasm; cytoplasmic membrane-bound vesicle; cytoplasm; dendritic spine; microtubule organizing center; cytoplasmic vesicle; trans-Golgi network; cell junction; cytosol
Molecular Function: protein binding; myosin binding; guanyl-nucleotide exchange factor activity; GABA receptor binding; ARF guanyl-nucleotide exchange factor activity
Biological Process: receptor recycling; Golgi to plasma membrane transport; protein transport; exocytosis; endosome organization and biogenesis; endomembrane organization; regulation of ARF protein signal transduction; positive regulation of tumor necrosis factor production; positive regulation of GTPase activity
Research Articles on ARFGEF2
1. interaction with the GABA(A)receptor beta subunits may play a role in the exocytosis and trafficking of assembled GABA(A) receptors to the cell surface
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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