Product Name
N-Acetyl Alpha-D-Glucosaminidase (NAGLU), ELISA Kit
Full Product Name
Human N-Acetyl Alpha-D-Glucosaminidase ELISA Kit (NAGLU)
Product Gene Name
NAGLU elisa kit
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Request Current Manual
3D Structure
ModBase 3D Structure for P54802
Assay Type
Sandwich ELISA
Samples
Cell Culture Supernatant, Serum, Plasma (EDTA, Citrate, Heparin)
Detection Range
0.312-20 ng/mL
Preparation and Storage
Store at 4 degree C.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of NAGLU elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for NAGLU purchase
MBS9137444 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the N-Acetyl Alpha-D-Glucosaminidase (NAGLU) ELISA Kit target analytes in
biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NAGLU. The ELISA analytical biochemical technique of the MBS9137444 kit is based on NAGLU antibody-NAGLU antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NAGLU antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NAGLU. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
NAGLU elisa kit
Product Overview: The ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of samples in cell culture supernatant, serum, plasma (EDTA, citrate, heparin).
Intended Use: This Human N-Acetyl Alpha-D-Glucosaminidase (NAGLU) ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Human N-Acetyl Alpha-D-Glucosaminidase (NAGLU) in the sample, this Human N-Acetyl Alpha-D-Glucosaminidase (NAGLU) ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus Human N-Acetyl Alpha-D-Glucosaminidase (NAGLU) concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI/Uniprot data below describe general gene information for NAGLU. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000254.2
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NCBI GenBank Nucleotide #
NM_000263.4
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UniProt Primary Accession #
P54802
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UniProt Related Accession #
P54802[Other Products]
Molecular Weight
82,266 Da
NCBI Official Full Name
alpha-N-acetylglucosaminidase
NCBI Official Synonym Full Names
N-acetyl-alpha-glucosaminidase
NCBI Official Symbol
NAGLU [Similar Products]
NCBI Official Synonym Symbols
NAG; CMT2V; MPS3B; UFHSD; MPS-IIIB
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NCBI Protein Information
alpha-N-acetylglucosaminidase
UniProt Protein Name
Alpha-N-acetylglucosaminidase
UniProt Synonym Protein Names
N-acetyl-alpha-glucosaminidase; NAG
Protein Family
Alpha-N-acetylglucosaminidase
UniProt Gene Name
NAGLU [Similar Products]
UniProt Synonym Gene Names
UFHSD1; NAG [Similar Products]
NCBI Summary for NAGLU
This gene encodes an enzyme that degrades heparan sulfate by hydrolysis of terminal N-acetyl-D-glucosamine residues in N-acetyl-alpha-D-glucosaminides. Defects in this gene are the cause of mucopolysaccharidosis type IIIB (MPS-IIIB), also known as Sanfilippo syndrome B. This disease is characterized by the lysosomal accumulation and urinary excretion of heparan sulfate. [provided by RefSeq, Jul 2008]
UniProt Comments for NAGLU
Involved in the degradation of heparan sulfate.CautionA MPS3B mutation at position 100 was erroneously reported (PubMed:9950362) as an amino acid change from Arg to His. The right amino acid change is from His to Arg.
Research Articles on NAGLU
1. findings show that the NAGLU protein consists of a precursor and a mature form and that in slowly progressing mucopolysaccharidosis type IIIB patients' fibroblasts only the precursor protein is present at 37 degrees ; culturing at lower temperatures resulted in the formation of the mature, enzymatically active form, due to higher mRNA levels and improved processing
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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