CRIPTO (TDGF1), Polyclonal Antibody

Teratocarcinoma-derived growth factor 1; Cripto-1 growth factor; CRGF; Epidermal growth factor-like cripto protein CR1; TDGF1; CRIPTO

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This CRIPTO (TDGF1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 18-48 amino acids from the N-terminal region of human CRIPTO (TDGF1).

Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.5 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-TDGF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

TDGF1 is expressed both in ES cells and during the early phases of embryo development, while in *****s it is reactivated in a wide range of epithelial cancers. This protein could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm. It is preferentially expressed in gastric and colorectal carcinomas compared to their normal counterparts.

Developmental Biology; Signal Transduction

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)

WB~~1:1000

Western blot analysis of CRIPTO (TDGF1) Antibody (N-term) in Jurkat cell line lysates (35ug/lane). TDGF1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

21169

teratocarcinoma-derived growth factor 1

teratocarcinoma-derived growth factor 1

Teratocarcinoma-derived growth factor 1

CRIPTO; CRGF  [Similar Products]

TDGF1_HUMAN

This gene encodes an epidermal growth factor-related protein that contains a cripto, FRL-1, and cryptic domain. The encoded protein is an extracellular, membrane-bound signaling protein that plays an essential role in embryonic development and tumor growth. Mutations in this gene are associated with forebrain defects. Pseudogenes of this gene are found on chromosomes 2, 3, 6, 8, 19 and X. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

TDGF1: Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm. Interacts with the activin type-1 receptor ACVR1B. Preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts. Expressed in breast and lung.

Protein type: Membrane protein, GPI anchor; Cell adhesion; Cell cycle regulation; Motility/polarity/chemotaxis; Membrane protein, peripheral

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: extrinsic to plasma membrane; Golgi apparatus; extracellular space; cell surface; perinuclear region of cytoplasm; apical plasma membrane; plasma membrane; nucleus; lipid raft

Molecular Function: protein binding; growth factor activity; receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; somatic stem cell maintenance; activation of MAPK activity; in utero embryonic development; heart development; regulation of signal transduction; gastrulation; Wnt receptor signaling pathway through beta-catenin; morphogenesis of a branching structure; cardiac muscle cell differentiation; BMP signaling pathway; positive regulation of peptidyl-tyrosine phosphorylation; embryonic development; peptidyl-serine phosphorylation; positive regulation of fibroblast proliferation; mammary gland development; cell migration during sprouting angiogenesis; determination of anterior/posterior axis, embryo; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; cell differentiation; negative regulation of transforming growth factor beta receptor signaling pathway; vasculogenesis; positive regulation of cell-matrix adhesion; positive regulation of cell migration; negative regulation of apoptosis

Parisi, S., et al., J. Cell Biol. 163(2):303-314 (2003).
Gray, P.C., et al., Proc. Natl. Acad. Sci. U.S.A. 100(9):5193-5198 (2003).
Yan, Y.T., et al., Mol. Cell. Biol. 22(13):4439-4449 (2002).
de la Cruz, J.M., et al., Hum. Genet. 110(5):422-428 (2002).
Dono, R., et al., Am. J. Hum. Genet. 49(3):555-565 (1991).

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.