Full Product Name
GAS1 siRNA (Human)
Product Synonym Names
Growth arrest-specific protein 1; GAS-1
Product Gene Name
GAS1 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P54826
Specificity
GAS1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human GAS1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of GAS1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
GAS1 sirna
siRNA to inhibit GAS1 expression using RNA interference
Applications Tested/Suitable for GAS1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for GAS1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_002039.2
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NCBI GenBank Nucleotide #
NM_002048.2
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UniProt Primary Accession #
P54826
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UniProt Secondary Accession #
Q6B086; B9EGM4[Other Products]
UniProt Related Accession #
P54826[Other Products]
Molecular Weight
35,693 Da
NCBI Official Full Name
growth arrest-specific protein 1
NCBI Official Synonym Full Names
growth arrest-specific 1
NCBI Official Symbol
GAS1 [Similar Products]
NCBI Protein Information
growth arrest-specific protein 1
UniProt Protein Name
Growth arrest-specific protein 1
Protein Family
Germacrene A synthase
UniProt Gene Name
GAS1 [Similar Products]
UniProt Synonym Gene Names
GAS-1 [Similar Products]
UniProt Entry Name
GAS1_HUMAN
NCBI Summary for GAS1
Growth arrest-specific 1 plays a role in growth suppression. GAS1 blocks entry to S phase and prevents cycling of normal and transformed cells. Gas1 is a putative tumor suppressor gene. [provided by RefSeq, Jul 2008]
UniProt Comments for GAS1
GAS1: Specific growth arrest protein involved in growth suppression. Blocks entry to S phase. Prevents cycling of normal and transformed cells.
Protein type: Membrane protein, GPI anchor
Chromosomal Location of Human Ortholog: 9q21.3-q22
Cellular Component: anchored to plasma membrane; plasma membrane; integral to membrane
Molecular Function: protein binding
Biological Process: developmental growth; axon guidance; camera-type eye development; cell fate commitment; outer ear morphogenesis; programmed cell death; cerebellum morphogenesis; palate development; embryonic cranial skeleton morphogenesis; middle ear morphogenesis; regulation of apoptosis; odontogenesis; negative regulation of smoothened signaling pathway; positive regulation of mesenchymal cell proliferation; positive regulation of smoothened signaling pathway; negative regulation of mitotic cell cycle; negative regulation of cell growth; embryonic digit morphogenesis; cell cycle arrest; positive regulation of epithelial cell proliferation; dorsoventral neural tube patterning; negative regulation of epithelial cell proliferation; eye morphogenesis; regulation of smoothened signaling pathway
Research Articles on GAS1
1. GAS1 is a novel endogenous inhibitor of glomerular mesangial cell proliferation.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
