CD33, Polyclonal Antibody

FLJ00391; SIGLEC-3; SIGLEC3; p67

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Affinity Purification

Store at -20 degree c. Avoid freeze / thaw cycles.

Small volumes of anti-CD33 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

CD33 is a type I transmembrane glycoprotein that is found on granulocyte and macrophage precursors in the bone marrow, but is absent from pluripotent stem cells. CD33 is also expressed on monocytes in peripheral blood. It is used as a marker to distinguish myelogenous leukemia cells from lymphoid or erythroid leukemias. CD33 may function as a sialic acid-dependent cell adhesion molecule.

Polyclonal

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

WB: 1:500 - 1:2000
IHC: 1:50 - 1:200

Western blot analysis of extracts of various cell lines, using CD33 Antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

Immunohistochemistry of paraffin-embdded human tonsil using CD33 antibody at dilution of 1:100 (40x lens).

Immunofluorescence analysis of THP-1 cells using CD33 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Calculated MW: 25kDa/33kDa/39kDa
Observed MW: 67kDa

CD33 molecule

myeloid cell surface antigen CD33; gp67; CD33 antigen (gp67); sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3

Myeloid cell surface antigen CD33

SIGLEC3; Siglec-3  [Similar Products]

CD33_HUMAN

CD33: Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro). Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: protein binding; receptor activity; carbohydrate binding

Biological Process: negative regulation of cell proliferation; cell-cell signaling; signal transduction; cell adhesion

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