Full Product Name
Monoclonal Antibody to hCD33 (Clone: wm53)
Product Synonym Names
Myeloid cell surface antigen CD33; Sialic acid-binding Ig-like lectin 3; Siglec-3; gp67
Product Gene Name
anti-CD33 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P20138
Purity/Purification
Purified
Protein G Chromatography
Form/Format
25 ug in 50 ul/100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Immunogen Information
Human AML cells were used as immunogen for this antibody.
Preparation and Storage
Store the antibody at 4 degree C, stable for 6 months.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
Other Notes
Small volumes of anti-CD33 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-CD33 antibody
Antibodies; Immunology
Applications Tested/Suitable for anti-CD33 antibody
Flow Cytometry (FC/FACS)
Application Notes for anti-CD33 antibody
Flow Cytometry: 0.5-1 ug/10^6 cells
Flow Cytometry (FC/FACS) of anti-CD33 antibody
Fig-1: Cell Surface flow analysis of hCD33 in PBMC using 0.5 ug/10^6 cells. Green represents isotype control ; red represents anti-hCD33 antibody. Goat anti-mouse PE conjugated secondary antibody was used.
NCBI/Uniprot data below describe general gene information for CD33. It may not necessarily be applicable to this product.
NCBI Accession #
P20138.2
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UniProt Primary Accession #
P20138
[Other Products]
UniProt Secondary Accession #
Q8TD24; B4E3P8; C9JEN7; F8WAL2[Other Products]
UniProt Related Accession #
P20138[Other Products]
Molecular Weight
25,293 Da
NCBI Official Full Name
Myeloid cell surface antigen CD33
NCBI Official Synonym Full Names
CD33 molecule
NCBI Official Symbol
CD33 [Similar Products]
NCBI Official Synonym Symbols
p67; SIGLEC3; SIGLEC-3
[Similar Products]
NCBI Protein Information
myeloid cell surface antigen CD33
UniProt Protein Name
Myeloid cell surface antigen CD33
UniProt Synonym Protein Names
Sialic acid-binding Ig-like lectin 3; Siglec-3; gp67; CD_antigen: CD33
Protein Family
Myeloid cell surface antigen
UniProt Gene Name
CD33 [Similar Products]
UniProt Synonym Gene Names
SIGLEC3; Siglec-3 [Similar Products]
UniProt Comments for CD33
CD33: Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro). Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Protein type: Cell adhesion; Membrane protein, integral
Chromosomal Location of Human Ortholog: 19q13.41
Cellular Component: external side of plasma membrane; integral to plasma membrane; plasma membrane
Molecular Function: protein binding; receptor activity
Biological Process: cell-cell signaling; negative regulation of cell proliferation; regulation of immune response; signal transduction
Research Articles on CD33
1. CD33-expressing microglia play a central role in the development of leukoencephalopathy in Nasu-Hakola disease brains
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
