Full Product Name
APOB siRNA (Mouse)
Product Synonym Names
Apolipoprotein B-100; Apo B-100
Product Gene Name
APOB sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for E9Q414
Specificity
APOB siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse APOB gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of APOB sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
APOB sirna
siRNA to inhibit APOB expression using RNA interference
Applications Tested/Suitable for APOB sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for APOB. It may not necessarily be applicable to this product.
NCBI Accession #
NP_033823.2
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NCBI GenBank Nucleotide #
NM_009693.2
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UniProt Primary Accession #
E9Q414
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UniProt Secondary Accession #
Q3UH74; Q497D8; Q61314; Q61318; Q8CGG8[Other Products]
UniProt Related Accession #
E9Q414[Other Products]
Molecular Weight
509,432 Da
NCBI Official Full Name
apolipoprotein B-100
NCBI Official Synonym Full Names
apolipoprotein B
NCBI Official Symbol
Apob [Similar Products]
NCBI Official Synonym Symbols
apob-48; AI315052; apob-100; Apo B-100
[Similar Products]
NCBI Protein Information
apolipoprotein B-100
UniProt Protein Name
Apolipoprotein B-100
Protein Family
Apolipoprotein
UniProt Gene Name
Apob [Similar Products]
UniProt Synonym Gene Names
Apo B-100; Apo B-48 [Similar Products]
UniProt Entry Name
APOB_MOUSE
NCBI Summary for APOB
This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100. Unlike the apoB-48 and apoB-100 structural equivalents in human, which are synthesized exclusively in the gut and liver, respectively, the mouse apoB-48 isoform is also found in mouse liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2179 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. [provided by RefSeq, Jul 2008]
UniProt Comments for APOB
APOB: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. Defects in APOB are a cause of familial hypobetalipoproteinemia type 1 (FHBL1). A disorder characterized by highly reduced plasma concentrations of low density lipoproteins, and dietary fat malabsorption. Clinical presentation may vary from no symptoms to severe gastrointestinal and neurological dysfunction similar to abetalipoproteinemia. Defects in APOB are a cause of familial ligand-defective apolipoprotein B-100 (FDB). FDB is a dominantly inherited disorder of lipoprotein metabolism leading to hypercholesterolemia and increased proneness to coronary artery disease (CAD). The plasma cholesterol levels are dramatically elevated due to impaired clearance of LDL particles by defective APOB/E receptors. Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.
Protein type: Secreted, signal peptide; Carrier; Secreted
Cellular Component: Golgi apparatus; extracellular space; chylomicron; cell soma; intracellular membrane-bound organelle; endoplasmic reticulum; cytoplasm; extracellular region; plasma membrane; vesicle membrane; actin cytoskeleton
Molecular Function: lipid transporter activity; heparin binding; low-density lipoprotein receptor binding; cholesterol transporter activity; phospholipid binding; lipid binding
Biological Process: steroid metabolic process; cholesterol metabolic process; nervous system development; lipoprotein catabolic process; cholesterol transport; in utero embryonic development; regulation of cholesterol biosynthetic process; cholesterol efflux; lipoprotein metabolic process; lipid transport; response to carbohydrate stimulus; post-embryonic development; sperm motility; cholesterol homeostasis; fertilization; lipoprotein biosynthetic process; transport; lipoprotein transport; triacylglycerol catabolic process; artery morphogenesis; spermatogenesis; lipid metabolic process; triacylglycerol mobilization; lipid catabolic process
Research Articles on APOB
1. Immunization with human apolipoprotein B100 (ApoB) resulted in four-fold increased accumulation of effector T cells in ApoB-containing matrigel
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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