Product Name
Melanophilin (Mlph), Polyclonal Antibody
Full Product Name
Goat anti-Melanophilin (mouse) Antibody
Product Synonym Names
2210418F23Rik; melanophilin; Mlph; ln; l1Rk3; Slac-2a; D1Wsu84e; l(1)-3Rk; 5031433I09Rik; leaden; AW228792; OTTMUSP00000025451; 2210418F23Rik antibody; melanophilin antibody; Mlph antibody; ln antibody; l1Rk3 antibody; Slac-2a antibody; D1Wsu84e antibody; l(1)-3Rk antibody; 5031433I09Rik antibody; leaden antibody; AW228792 antibody; OTTMUSP00000025451 antibody; Melanophilin; Melanophilin (mouse)
Product Gene Name
anti-Mlph antibody
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Antibody/Peptide Pairs
Melanophilin peptide (MBS425662) is used for blocking the activity of Melanophilin antibody (MBS421445)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
ARHIFAKPVM AQQP
Species Reactivity
Tested: Mouse; Expected from sequence similarity: Mouse, Rat
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-ARHIFAKPVMAQQP, from the C Terminus of the protein sequence according to NP_443748.2.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-Mlph antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-Mlph antibody
Peptide ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), GST-pull down
Application Notes for anti-Mlph antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 80 kDa band observed in Mouse Melanocyte extracts. Recommended for use at 0.5-2ug/ml. Please note that this product may require overnight incubation of primary and high protein loads.
Immunofluorescence: Successfully used at 0.5ug/ml to stain mouse melanocytes, see picture. See also publication Figueiredo AC et al. , J Biol Chem.2008 Aug 22;283(34): 23209-16. (PMID: 18559336).
Western Blot (WB) of anti-Mlph antibody
Staining (0.5ug/ml) of total melanocyte PNS (200ug per lane) of wild-type and leaden (Mlph null) mice. The upper band may represent the stacking-separating gel interface. Data kindly provided by A N Hume, Imperial College.
Immunofluorescence (IF) of anti-Mlph antibody
Wild-type C57bl6 derived melanocytes were stained (0.5ug/ml) and detected with Alexa568 conjugated donkey anti-sheep second antibodies. Brightfield image shows melanosome distribution. Data kindly provided by A N Hume, Imperial College.
NCBI/Uniprot data below describe general gene information for Mlph. It may not necessarily be applicable to this product.
NCBI Accession #
NP_443748.2
[Other Products]
NCBI GenBank Nucleotide #
NM_053015.3
[Other Products]
UniProt Secondary Accession #
Q3UFV7; Q99N53[Other Products]
UniProt Related Accession #
Q91V27[Other Products]
Molecular Weight
65,052 Da
NCBI Official Full Name
melanophilin
NCBI Official Synonym Full Names
melanophilin
NCBI Official Symbol
Mlph [Similar Products]
NCBI Official Synonym Symbols
ln; l1Rk3; Slac-2a; AW228792; D1Wsu84e; l(1)-3Rk; 2210418F23Rik; 5031433I09Rik
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NCBI Protein Information
melanophilin
UniProt Protein Name
Melanophilin
UniProt Synonym Protein Names
Exophilin-3; Leaden protein; Slp homolog lacking C2 domains a; SlaC2-a; Synaptotagmin-like protein 2a
Protein Family
Melanophilin
UniProt Gene Name
Mlph [Similar Products]
UniProt Synonym Gene Names
Ln; Slac2a; SlaC2-a [Similar Products]
UniProt Entry Name
MELPH_MOUSE
UniProt Comments for Mlph
MLPH: Rab effector protein involved in melanosome transport. Serves as link between melanosome-bound RAB27A and the motor protein MYO5A. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3). GS3 is a rare autosomal recessive disorder characterized by pigmentary dilution of the skin and hair, the presence of large clumps of pigment in hair shafts, and an accumulation of melanosomes in melanocytes, without other clinical manifestations. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Adaptor/scaffold
Cellular Component: actin cytoskeleton; cortical actin cytoskeleton; dendrite; melanosome; microtubule organizing center; perinuclear region of cytoplasm; stress fiber
Molecular Function: actin binding; metal ion binding; microtubule plus-end binding; myosin binding; myosin V binding; protein binding; Rab GTPase binding
Biological Process: intracellular protein transport; melanocyte differentiation; melanosome localization; pigmentation; protein targeting
Research Articles on Mlph
1. The results showed that Rab27A(Q78L) is unable to localize on mature melanosomes and that its inhibitory activity on melanosome transport is completely dependent on its binding to the Rab27A effector Slac2-a/melanophilin.
Precautions
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Disclaimer
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