Chitotriosidase, Assay Kit

For Research Use Only. Not for use in diagnostic procedures.

Store at -20 degree C for 12 months.

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Small volumes of CHIT1 assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Background/Introduction: Chitotriosidase (CHIT1 or chitinase-1), is a mammalian enzyme belonging to glycosyl hydrolase-18 family (EC 3.2.1.14), which can hydrolyze the beta-(1, 4)-linkage between the adjacent N-acetyl glucosamine residues of chitin. CHIT1 is synthesized exclusively by activated macrophages both in normal and inflammatory conditions and, as a host defense factor, CHIT1 plays a pivotal role in infectious disease such as malaria and fungi infectious. Increased CHIT1 activity has also been found in patients suffering diseases such as bronchial asthma and atherosclerosis. CHIT1 has been used as a valuable diagnostic biomarker for monitoring the therapeutic efficacy of treatments for Gaucher's disease or beta-glucocerebrosidase deficiency. Chitotriosidase Activity Assay, utilizes a fluorogenic substrate that can be hydrolyzed by chitinase and a set of proprietary assay buffers that can distinguish specific CHIT1 activity from other hydrolases including Acidic Mammalian Chitinase. The kit provides a simple, specific, sensitive assay that can detect as low as 0.2 mU/ml of CHIT1 in a variety of biological samples.

Carbohydrate Metabolism

Measurement of Chitotriosidase activity in various biological samples and purified/crude enzyme preparations

49,613 Da[Similar Products]

chitinase 1

chitotriosidase-1

Chitotriosidase-1

Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Several alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jan 2012]

CHIT1: Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens. Isoform 3 has no enzymatic activity. Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - amino sugar and nucleotide sugar; EC 3.2.1.14; Hydrolase; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: extracellular region; extracellular space

Molecular Function: chitin binding; chitinase activity; endochitinase activity; hydrolase activity, hydrolyzing O-glycosyl compounds

Biological Process: carbohydrate metabolic process; chitin catabolic process; neutrophil degranulation; response to bacterium

Disease: Chitotriosidase Deficiency

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