Product Name
Melanophilin (MLPH), ELISA Kit
Full Product Name
Human Melanophilin (MLPH) ELISA Kit
Product Gene Name
MLPH elisa kit
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sample Manual Insert
Download Sample PDF Manual View Sample PDF Manual
Request for Current Manual Insert
Request Current Manual
3D Structure
ModBase 3D Structure for Q91V27
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
10ng/L - 3000ng/L
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of MLPH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for MLPH purchase
MBS167347 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Melanophilin (MLPH) ELISA Kit target analytes in
biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MLPH. The ELISA analytical biochemical technique of the MBS167347 kit is based on MLPH antibody-MLPH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MLPH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MLPH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
MLPH elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Melanophilin (also known as MLPH) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human MLPH antibody. MLPH present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human MLPH
Antibody is added and binds to MLPH in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MLPH antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human MLPH. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Typical Testing Data/Standard Curve (for reference only) of MLPH elisa kit
NCBI/Uniprot data below describe general gene information for MLPH. It may not necessarily be applicable to this product.
NCBI Accession #
NP_443748.2
[Other Products]
NCBI GenBank Nucleotide #
NM_053015.3
[Other Products]
UniProt Primary Accession #
Q91V27
[Other Products]
UniProt Secondary Accession #
Q3UFV7; Q99N53[Other Products]
UniProt Related Accession #
Q91V27[Other Products]
Molecular Weight
65,052 Da
NCBI Official Full Name
melanophilin
NCBI Official Synonym Full Names
melanophilin
NCBI Official Symbol
Mlph [Similar Products]
NCBI Official Synonym Symbols
ln; l1Rk3; Slac-2a; AW228792; D1Wsu84e; l(1)-3Rk; 2210418F23Rik; 5031433I09Rik
[Similar Products]
NCBI Protein Information
melanophilin
UniProt Protein Name
Melanophilin
UniProt Synonym Protein Names
Exophilin-3; Leaden protein; Slp homolog lacking C2 domains a; SlaC2-a; Synaptotagmin-like protein 2a
Protein Family
Melanophilin
UniProt Gene Name
Mlph [Similar Products]
UniProt Synonym Gene Names
Ln; Slac2a; SlaC2-a [Similar Products]
UniProt Comments for MLPH
Rab effector protein involved in melanosome transport. Serves as link between melanosome-bound RAB27A and the motor protein MYO5A.
Research Articles on MLPH
1. When actin and microtubules were present simultaneously, melanophilin's phosphorylation state enforced track selection of the Rab27a/melanophilin/myosin Va transport complex.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
