Product Name
Vitronectin (VTN), Monoclonal Antibody
Full Product Name
MAb to Vitronectin
Product Synonym Names
Monoclonal Antibody to Human Vitronectin (VN)
Product Gene Name
anti-VTN antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 17; NC_000017.10 (26694298..26697373, complement). Location: 17q11
3D Structure
ModBase 3D Structure for P04004
Host
Host: Mouse. Hybridization of NS-1myeloma cells with spleen cells from Balb/c mice.
Source: Cell Culture
Specificity
Vitronectin
Antibody binds human Vitronectin in solid-phase ELISA and immunoblotting procedures. Binds plasma Vitronectin when coupled to sepharose.
Purity/Purification
>98% pure. Protein G chromatography
Form/Format
Purified, Lyophilized
Reconstitute with distilled water. Centrifuge to remove insoluble material
Concentration
1mg/ml (prior to lyophilization) (lot specific)
Affinity Constant
Not determined
Buffer
Lyophilized from 10mM Sodium phosphate, 0.15M Sodium chloride, pH 7.4
Preservative
0.01% Sodium azide
Important Note
Centrifuge before opening to ensure complete recovery of vial contents.
Warnings
This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1-1.0%. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
Preparation and Storage
Store lyophilized product at -20 degree C. After reconstitution, store (up to 30 days) at 2 to 8 degree C or aliquot and store at -20 degree C. Avoid multiple freeze/thaw cycles.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of anti-VTN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-VTN antibody
Monoclonal Antibodies to Platelets and Hemostasis Antigens
Application Notes for anti-VTN antibody
Detection of human Vitronectin by ELISA or immunoblotting. Purification of plasma Vitronectin when coupled to sepharose. Recommended use: 1-5ug/ml. Each laboratory should determine an optimum working titer for use in its particular application. Other appl
NCBI/Uniprot data below describe general gene information for VTN. It may not necessarily be applicable to this product.
NCBI Accession #
AAK60270.1
[Other Products]
UniProt Primary Accession #
P04004
[Other Products]
UniProt Secondary Accession #
P01141; Q9BSH7; B2R7G0[Other Products]
UniProt Related Accession #
P04004[Other Products]
Molecular Weight
54,306 Da[Similar Products]
NCBI Official Full Name
vitronectin
NCBI Official Synonym Full Names
vitronectin
NCBI Official Symbol
VTN [Similar Products]
NCBI Official Synonym Symbols
VN; V75; VNT
[Similar Products]
NCBI Protein Information
vitronectin; epibolin; S-protein; somatomedin B; OTTHUMP00000163323; complement S-protein; serum spreading factor; serum-spreading factor
UniProt Protein Name
Vitronectin
UniProt Synonym Protein Names
S-protein; Serum-spreading factor; V75
Protein Family
Vitronectin
UniProt Gene Name
VTN [Similar Products]
UniProt Entry Name
VTNC_HUMAN
NCBI Summary for VTN
The protein encoded by this gene is a member of the pexin family. It is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond. [provided by RefSeq]
UniProt Comments for VTN
Function: Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
Subunit structure: Exists in two forms: a single chain 75 kDa form (V75) and a clipped form composed of two chains (65 kDa and 10 kDa) (V65+V10) which are held together by a disulfide bond. Interacts with SERPINE1/PAI1 and insulin. Ref.9 Ref.10 Ref.15
Subcellular location: Secreted › extracellular space.
Tissue specificity: Plasma. Ref.25
Domain: The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.
Post-translational modification: Sulfated on 2 tyrosine residues.N- and O-glycosylated
By similarity. Ref.18 Ref.19 Ref.22 Ref.24Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.Phosphorylation sites are present in the extracelllular medium.
Sequence similarities: Contains 4 hemopexin-like domains.Contains 1 SMB (somatomedin-B) domain.
Research Articles on VTN
1. PAI-2 is capable of inhibiting uPA in the presence of PAI-1, particularly on adherent cells in the presence of vitronectin
Precautions
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Disclaimer
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