Neurogenic Differentiation 1 (NEUROD1), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C

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Small volumes of NEUROD1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9354057 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Neurogenic Differentiation 1 (NEUROD1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NEUROD1. The ELISA analytical biochemical technique of the MBS9354057 kit is based on NEUROD1 antibody-NEUROD1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NEUROD1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NEUROD1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

39,920 Da

neuronal differentiation 1

neurogenic differentiation factor 1

Neurogenic differentiation factor 1

BHLHA3; NEUROD; NeuroD; NeuroD1; bHLHa3  [Similar Products]

NDF1_HUMAN

This gene encodes a member of the NeuroD family of basic helix-loop-helix (bHLH) transcription factors. The protein forms heterodimers with other bHLH proteins and activates transcription of genes that contain a specific DNA sequence known as the E-box. It regulates expression of the insulin gene, and mutations in this gene result in type II diabetes mellitus. [provided by RefSeq, Jul 2008]

NEUROD1: Acts as a transcriptional activator: mediates transcriptional activation by binding to E box-containing promoter consensus core sequences 5'-CANNTG-3'. Associates with the p300/CBP transcription coactivator complex to stimulates transcription of the secretin gene as well as the gene encoding the cyclin-dependent kinase inhibitor p21Cip1. Contributes to the regulation of several cell differentiation pathways, like those that promote the formation of early retinal ganglion cells, inner ear sensory neurons, granule cells forming either the cerebellum or the dentate gyrus cell layer of the hippocampus, endocrine islet cells of the pancreas and enteroendocrine cells of the small intestine. Together with PAX6 or SIX3, is required for the regulation of amacrine cell fate specification. Also required for dendrite morphogenesis and maintenance in the cerebellar cortex. Associates with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis. Interacts (via helix-loop-helix motif domain) with EP300 (via C-terminus). Heterodimer with TCF3/E47; the heterodimer is inhibited in presence of ID2, but not NR0B2, to E- box element. Efficient DNA-binding requires dimerization with another bHLH protein. Interacts with RREB1. Interacts with EP300; the interaction is inhibited by NR0B2. Interacts with TCF3; the interaction is inhibited by ID2.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 2q32

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function: chromatin binding; protein binding; protein heterodimerization activity; sequence-specific DNA binding; transcription coactivator activity; transcription factor activity; transcription factor binding

Biological Process: anterior/posterior pattern formation; cerebellum development; dentate gyrus development; embryonic organ morphogenesis; endocrine pancreas development; glucose homeostasis; inner ear development; insulin secretion; negative regulation of JAK-STAT cascade; neurogenesis; neuron development; nitric oxide mediated signal transduction; nucleocytoplasmic transport; positive regulation of apoptosis; positive regulation of cell differentiation; positive regulation of neuron differentiation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of insulin secretion; response to drug; response to glucose stimulus; transcription from RNA polymerase II promoter

Disease: Diabetes Mellitus, Noninsulin-dependent; Maturity-onset Diabetes Of The Young, Type 6

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