PRKAR2A, Polyclonal Antibody

cAMP-dependent protein kinase type II-alpha regulatory subunit; PRKAR2A; PKR2; PRKAR2

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

>95%, Protein G purified

Liquid

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Small volumes of anti-PRKAR2A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:200

Western Blot
Positive WB detected in: Hela whole cell lysate,MCF-7 whole cell lysate,PC3 whole cell lysate
All lanes: PRKAR2A antibody at 2.5ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 46,44 KDa
Observed band size: 46 KDa

Immunohistochemistry of paraffin-embedded human lymph node tissue using MBS7052305 at dilution of 1:100

Immunohistochemistry of paraffin-embedded human glioma cancer using MBS7052305 at dilution of 1:100

Immunofluorescent analysis of HepG2 cells using MBS7052305 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

43,067 Da

protein kinase cAMP-dependent type II regulatory subunit alpha

cAMP-dependent protein kinase type II-alpha regulatory subunit

cAMP-dependent protein kinase type II-alpha regulatory subunit

PKR2; PRKAR2  [Similar Products]

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER). [provided by RefSeq, Jul 2008]

PKAR2A: a regulatory subunit of cAMP-regulated protein kinase. The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.

Protein type: Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: AMP-activated protein kinase complex; centrosome; cytoplasm; cytosol; focal adhesion; membrane; plasma membrane

Molecular Function: cAMP-dependent protein kinase inhibitor activity; cAMP-dependent protein kinase regulator activity; protein binding; ubiquitin protein ligase binding

Biological Process: activation of protein kinase A; blood coagulation; renal water homeostasis

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