Product Name
RFWD3, siRNA
Full Product Name
RFWD3 siRNA (Mouse)
Product Synonym Names
RNF201; E3 ubiquitin-protein ligase RFWD3; RING finger and WD repeat domain-containing protein 3; RING finger protein 201
Product Gene Name
RFWD3 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8CIK8
Specificity
RFWD3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse RFWD3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of RFWD3 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
RFWD3 sirna
siRNA to inhibit RFWD3 expression using RNA interference
Applications Tested/Suitable for RFWD3 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for RFWD3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_666330.2
[Other Products]
NCBI GenBank Nucleotide #
NM_146218.4
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UniProt Primary Accession #
Q8CIK8
[Other Products]
UniProt Secondary Accession #
Q3UV90; Q6PJA8; Q8R2T9; Q8R351[Other Products]
UniProt Related Accession #
Q8CIK8[Other Products]
Molecular Weight
84,312 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase RFWD3
NCBI Official Synonym Full Names
ring finger and WD repeat domain 3
NCBI Official Symbol
Rfwd3 [Similar Products]
NCBI Official Synonym Symbols
BC027246
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NCBI Protein Information
E3 ubiquitin-protein ligase RFWD3
UniProt Protein Name
E3 ubiquitin-protein ligase RFWD3
UniProt Synonym Protein Names
RING finger and WD repeat domain-containing protein 3; RING finger protein 201
Protein Family
E3 ubiquitin-protein ligase
UniProt Gene Name
Rfwd3 [Similar Products]
UniProt Synonym Gene Names
Rnf201 [Similar Products]
UniProt Entry Name
RFWD3_MOUSE
UniProt Comments for RFWD3
RFWD3: E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53. Plays a role in RPA-mediated DNA damage signaling and repair.
Protein type: Ubiquitin conjugating system; EC 6.3.2.-; EC 6.3.2.19; Ubiquitin ligase
Cellular Component: cytoplasm; nucleus
Molecular Function: zinc ion binding; p53 binding; coenzyme F420-2 alpha-glutamyl ligase activity; metal ion binding; UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity; ubiquitin-protein ligase activity; ribosomal S6-glutamic acid ligase activity; coenzyme F420-0 gamma-glutamyl ligase activity; ligase activity
Biological Process: G1 DNA damage checkpoint; protein ubiquitination; response to ionizing radiation; DNA repair; response to DNA damage stimulus
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
