Product Name
NEUROD1, siRNA
Full Product Name
NEUROD1 siRNA (Human)
Product Synonym Names
BHLHA3; NEUROD; Neurogenic differentiation factor 1; NeuroD; NeuroD1; Class A basic helix-loop-helix protein 3; bHLHa3
Product Gene Name
NEUROD1 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q13562
Specificity
NEUROD1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NEUROD1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of NEUROD1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
NEUROD1 sirna
siRNA to inhibit NEUROD1 expression using RNA interference
Applications Tested/Suitable for NEUROD1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for NEUROD1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_002491.2
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NCBI GenBank Nucleotide #
NM_002500.4
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UniProt Primary Accession #
Q13562
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UniProt Secondary Accession #
O00343; Q13340; Q5U095; Q96TH0; Q99455; Q9UEC8; B2R9I8; F1T0E1[Other Products]
UniProt Related Accession #
Q13562[Other Products]
Molecular Weight
39,920 Da
NCBI Official Full Name
neurogenic differentiation factor 1
NCBI Official Synonym Full Names
neuronal differentiation 1
NCBI Official Symbol
NEUROD1 [Similar Products]
NCBI Official Synonym Symbols
BETA2; BHF-1; MODY6; NEUROD; bHLHa3
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NCBI Protein Information
neurogenic differentiation factor 1
UniProt Protein Name
Neurogenic differentiation factor 1
UniProt Synonym Protein Names
Class A basic helix-loop-helix protein 3; bHLHa3
UniProt Gene Name
NEUROD1 [Similar Products]
UniProt Synonym Gene Names
BHLHA3; NEUROD; NeuroD; NeuroD1; bHLHa3 [Similar Products]
UniProt Entry Name
NDF1_HUMAN
NCBI Summary for NEUROD1
This gene encodes a member of the NeuroD family of basic helix-loop-helix (bHLH) transcription factors. The protein forms heterodimers with other bHLH proteins and activates transcription of genes that contain a specific DNA sequence known as the E-box. It regulates expression of the insulin gene, and mutations in this gene result in type II diabetes mellitus. [provided by RefSeq, Jul 2008]
UniProt Comments for NEUROD1
NEUROD1: Acts as a transcriptional activator: mediates transcriptional activation by binding to E box-containing promoter consensus core sequences 5'-CANNTG-3'. Associates with the p300/CBP transcription coactivator complex to stimulates transcription of the secretin gene as well as the gene encoding the cyclin-dependent kinase inhibitor p21Cip1. Contributes to the regulation of several cell differentiation pathways, like those that promote the formation of early retinal ganglion cells, inner ear sensory neurons, granule cells forming either the cerebellum or the dentate gyrus cell layer of the hippocampus, endocrine islet cells of the pancreas and enteroendocrine cells of the small intestine. Together with PAX6 or SIX3, is required for the regulation of amacrine cell fate specification. Also required for dendrite morphogenesis and maintenance in the cerebellar cortex. Associates with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis. Interacts (via helix-loop-helix motif domain) with EP300 (via C-terminus). Heterodimer with TCF3/E47; the heterodimer is inhibited in presence of ID2, but not NR0B2, to E- box element. Efficient DNA-binding requires dimerization with another bHLH protein. Interacts with RREB1. Interacts with EP300; the interaction is inhibited by NR0B2. Interacts with TCF3; the interaction is inhibited by ID2.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 2q32
Cellular Component: nucleoplasm; cytoplasm; nucleus
Molecular Function: protein binding; sequence-specific DNA binding; protein heterodimerization activity; double-stranded DNA binding; transcription coactivator activity; chromatin binding; transcription factor binding; transcription factor activity
Biological Process: response to drug; dentate gyrus development; transcription, DNA-dependent; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; nucleocytoplasmic transport; glucose homeostasis; endocrine pancreas development; anterior/posterior pattern formation; embryonic organ morphogenesis; neurogenesis; insulin secretion; response to glucose stimulus; cerebellum development; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of cell differentiation; positive regulation of neuron differentiation; regulation of insulin secretion; inner ear development; nitric oxide mediated signal transduction; negative regulation of JAK-STAT cascade
Disease: Maturity-onset Diabetes Of The Young, Type 6; Diabetes Mellitus, Noninsulin-dependent
Research Articles on NEUROD1
1. this study concludes that the novel mechanism would regulate the expression of ALK in neuroblastoma and that NeuroD1 should be significantly involved in neuroblastoma tumorigenesis.
Precautions
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Disclaimer
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