Vitamin K epoxide reductase complex subunit 1, ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

This assay recognizes recombinant and natural Bovine VKORC1. No significant cross-reactivity or interference was observed.

For long term storage, please store the entire kit at -20 degree C.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of VKORC1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS288306 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Vitamin K epoxide reductase complex subunit 1 (VKORC1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing VKORC1. The ELISA analytical biochemical technique of the MBS288306 kit is based on VKORC1 antibody-VKORC1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect VKORC1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, VKORC1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to VKORC1, During the reaction, VKORC1 in the sample or standard competes with a fixed amount of biotin-labeled VKORC1 for sites on a pre-coated Monoclonal antibody specific to VKORC1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of VKORC1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

17,967 Da

vitamin K epoxide reductase complex subunit 1

Vitamin K epoxide reductase complex subunit 1

VKOR1_BOVIN

Involved in vitamin K metabolism. Catalytic subunit of the vitamin K epoxide reductase (VKOR) complex which reduces inactive vitamin K 2,3-epoxide to active vitamin K. Vitamin K is required for the gamma-carboxylation of various proteins, including clotting factors, and is required for normal blood coagulation, but also for normal bone development ().

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