Product Name
ATOH1, Polyclonal Antibody
Popular Item
Full Product Name
ATOH1 Polyclonal Antibody
Product Synonym Names
ATH1; HATH1; MATH-1; bHLHa14
Product Gene Name
anti-ATOH1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q92858
Species Reactivity
Human, Mouse
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human ATOH1
Calculated Molecular Weight
38kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Other Notes
Small volumes of anti-ATOH1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ATOH1 antibody
This protein belongs to the basic helix-loop-helix (BHLH) family of transcription factors. It activates E-box dependent transcription along with E47.
Product Categories/Family for anti-ATOH1 antibody
Polyclonal
Applications Tested/Suitable for anti-ATOH1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-ATOH1 antibody
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Western Blot (WB) of anti-ATOH1 antibody
Western blot analysis of extracts of various cell lines, using ATOH1 Antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunofluorescence (IF) of anti-ATOH1 antibody
Immunofluorescence analysis of U2OS cells using ATOH1 antibody. Blue: DAPI for nuclear staining.
NCBI/Uniprot data below describe general gene information for ATOH1. It may not necessarily be applicable to this product.
NCBI Accession #
Q92858.1
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UniProt Primary Accession #
Q92858
[Other Products]
UniProt Secondary Accession #
Q14CT9[Other Products]
UniProt Related Accession #
Q92858[Other Products]
NCBI Official Full Name
Protein atonal homolog 1
NCBI Official Synonym Full Names
atonal homolog 1 (Drosophila)
NCBI Official Symbol
ATOH1 [Similar Products]
NCBI Official Synonym Symbols
ATH1; HATH1; MATH-1; bHLHa14
[Similar Products]
NCBI Protein Information
protein atonal homolog 1; helix-loop-helix protein hATH-1; class A basic helix-loop-helix protein 14
UniProt Protein Name
Protein atonal homolog 1
UniProt Synonym Protein Names
Class A basic helix-loop-helix protein 14; bHLHa14; Helix-loop-helix protein hATH-1; hATH1
Protein Family
Protein atonal
UniProt Gene Name
ATOH1 [Similar Products]
UniProt Synonym Gene Names
ATH1; BHLHA14; bHLHa14; hATH1 [Similar Products]
UniProt Entry Name
ATOH1_HUMAN
NCBI Summary for ATOH1
This protein belongs to the basic helix-loop-helix (BHLH) family of transcription factors. It activates E-box dependent transcription along with E47. [provided by RefSeq, Jul 2008]
UniProt Comments for ATOH1
ATOH1: a conserved transcription factor that regulates secretory cell differentiation in colonic epithelium. Activates E box-dependent transcription in collaboration with TCF3/E47, but the activity is completely antagonized by the negative regulator of neurogenesis HES1. May play a role in the differentiation of subsets of neural cells by activating E box- dependent transcription . Efficient DNA binding requires dimerization with another bHLH protein. Loss of ATOH1 expression in the colon of mice initiates colon cancer. ATOH1 has been silenced in most human colon cancers, and reactivation of this gene in cultured human colon cancer cells is anti-proliferative and induces apoptosis.
Protein type: Cell development/differentiation; DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 4q22
Cellular Component: nucleus
Molecular Function: protein dimerization activity; chromatin DNA binding; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; axon guidance; inner ear morphogenesis; central nervous system development; auditory receptor cell fate determination; neuron migration; positive regulation of transcription from RNA polymerase II promoter; positive regulation of auditory receptor cell differentiation; cerebral cortex development; auditory receptor cell fate specification; positive regulation of neuron differentiation; negative regulation of apoptosis
Research Articles on ATOH1
1. Expression of Hath1, Muc2, cyclin D1 and p27 in the xenograft tumors was also detected.
Precautions
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Disclaimer
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