ATOH1, Polyclonal Antibody

ATH1; HATH1; MATH-1; bHLHa14

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Affinity Purification

Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Manufactured in an ISO 9001:2015 Certified Laboratory.

Small volumes of anti-ATOH1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This protein belongs to the basic helix-loop-helix (BHLH) family of transcription factors. It activates E-box dependent transcription along with E47.

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

WB: 1:500-1:2000, IHC: 1:50-1:200, IF: 1:50-1:200

Western blot analysis of extracts of various cell lines, using ATOH1 antibody.

Immunofluorescence analysis of U20S cell using ATOH1 antibody. Blue: DAPI for nuclear staining.

Calculated MW: 38kDa

atonal bHLH transcription factor 1

protein atonal homolog 1

Protein atonal homolog 1

ATH1; BHLHA14; bHLHa14; hATH1  [Similar Products]

ATOH1_HUMAN

This protein belongs to the basic helix-loop-helix (BHLH) family of transcription factors. It activates E-box dependent transcription along with E47. [provided by RefSeq, Jul 2008]

ATOH1: a conserved transcription factor that regulates secretory cell differentiation in colonic epithelium. Activates E box-dependent transcription in collaboration with TCF3/E47, but the activity is completely antagonized by the negative regulator of neurogenesis HES1. May play a role in the differentiation of subsets of neural cells by activating E box- dependent transcription . Efficient DNA binding requires dimerization with another bHLH protein. Loss of ATOH1 expression in the colon of mice initiates colon cancer. ATOH1 has been silenced in most human colon cancers, and reactivation of this gene in cultured human colon cancer cells is anti-proliferative and induces apoptosis.

Protein type: Transcription factor; DNA-binding; Cell development/differentiation

Chromosomal Location of Human Ortholog: 4q22

Cellular Component: nucleus

Molecular Function: chromatin DNA binding; protein dimerization activity; transcription factor activity

Biological Process: auditory receptor cell fate determination; auditory receptor cell fate specification; axon guidance; central nervous system development; cerebral cortex development; inner ear morphogenesis; negative regulation of apoptosis; neuron migration; Notch signaling pathway; positive regulation of auditory receptor cell differentiation; positive regulation of neuron differentiation; positive regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter

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