Electrogenic sodium bicarbonate cotransporter 1 (SLC4A4), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

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Small volumes of SLC4A4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7247510 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Electrogenic sodium bicarbonate cotransporter 1 (SLC4A4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SLC4A4. The ELISA analytical biochemical technique of the MBS7247510 kit is based on SLC4A4 antibody-SLC4A4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SLC4A4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SLC4A4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Neurobiology

123,181 Da

solute carrier family 4 (sodium bicarbonate cotransporter), member 4

electrogenic sodium bicarbonate cotransporter 1; kNBC1; Na(+)/HCO3(-) cotransporter; solute carrier family 4, sodium bicarbonate cotransporter, member 5; solute carrier family 4, sodium bicarbonate cotransporter, member 4, brain type; sodium bicarbonate cotransporter 1 (sodium bicarbonate cotransporter, kidney; sodium bicarbonate cotransporter, pancreas)

Electrogenic sodium bicarbonate cotransporter 1

NBC; NBC1; NBCE1; Sodium bicarbonate cotransporter  [Similar Products]

S4A4_HUMAN

This gene encodes a sodium bicarbonate cotransporter (NBC) involved in the regulation of bicarbonate secretion and absorption and intracellular pH. Mutations in this gene are associated with proximal renal tubular acidosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]

SLC4A4: Electrogenic sodium/bicarbonate cotransporter with a Na(+):HCO3(-) stoichiometry varying from 1:2 to 1:3. May regulate bicarbonate influx/efflux at the basolateral membrane of cells and regulate intracellular pH. Defects in SLC4A4 are the cause of proximal renal tubular acidosis with ocular abnormalities (pRTA-OA); also known as renal tubular acidosis II. Caused by an impairment of bicarbonate absorption in the proximal tubule, proximal renal tubular acidosis (pRTA) is characterized by a decreased renal HCO3(-) threshold. pRTA-OA is an extremely rare autosomal recessive syndrome characterized by short stature, profound pRTA, mental retardation, bilateral glaucoma, cataracts and bandkeratopathy. Loss of interaction with and stimulation by CA4 is the cause of retinitis pigmentosa type 17 (RP17). Belongs to the anion exchanger (TC 2.A.31) family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, SLC family; Transporter; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 4q21

Cellular Component: integral to plasma membrane; basolateral plasma membrane; plasma membrane

Molecular Function: protein binding; inorganic anion exchanger activity; sodium:bicarbonate symporter activity

Biological Process: transport; bicarbonate transport; regulation of intracellular pH; ion transport; transmembrane transport

Disease: Renal Tubular Acidosis, Proximal, With Ocular Abnormalities And Mental Retardation

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