ITGB4, Polyclonal Antibody

Integrin beta-4; GP150; CD104; ITGB4

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

>95%, Protein G purified

Liquid

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Small volumes of anti-ITGB4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility. ITGA6: ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed: 20682778). ITGA6: ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed: 22351760).

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

WB: 1: 500-1: 5000
IHC: 1: 20-1: 200
IF: 1: 50-1: 200

Western Blot
Positive WB detected in:Mouse brain tissue
All lanes: ITGB4 antibody at 3.5ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 203,196,201,195,107 kDa
Observed band size: 203 kDa

Immunohistochemistry of paraffin-embedded human placenta tissue using MBS7048492 at dilution of 1:100

Immunohistochemistry of paraffin-embedded human pancreatic cancer using MBS7048492 at dilution of 1:100

Immunofluorescent analysis of A549 cells using MBS7048492 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

106,779 Da

integrin subunit beta 4

integrin beta-4

Integrin beta-4

ITB4_HUMAN

Integrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

ITGB4: integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells. Five alternatively spliced isoforms have been described.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc.

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: cell junction; cell surface; hemidesmosome; leading edge; nucleus; plasma membrane; receptor complex

Molecular Function: G-protein-coupled receptor binding; insulin-like growth factor I binding; protein binding

Biological Process: autophagy; cell motility involved in cell locomotion; cell-matrix adhesion; extracellular matrix organization and biogenesis; gut development; hemidesmosome assembly; mesodermal cell differentiation; response to wounding; skin development

Disease: Epidermolysis Bullosa Junctionalis With Pyloric Atresia; Epidermolysis Bullosa Simplex, Localized; Epidermolysis Bullosa, Junctional, Non-herlitz Type

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