Product Name
ITGB4, siRNA
Full Product Name
ITGB4 siRNA (Mouse)
Product Synonym Names
Integrin beta-4; CD antigen CD104
Product Gene Name
ITGB4 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for A2A863
Specificity
ITGB4 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ITGB4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ITGB4 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ITGB4 sirna
siRNA to inhibit ITGB4 expression using RNA interference
Applications Tested/Suitable for ITGB4 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ITGB4. It may not necessarily be applicable to this product.
NCBI Accession #
XP_006532635.1
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NCBI GenBank Nucleotide #
XM_006532572.2
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UniProt Primary Accession #
A2A863
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UniProt Secondary Accession #
Q6PCS0; Q8R3J1; Q91W15; A2A865; A2A866[Other Products]
UniProt Related Accession #
A2A863[Other Products]
Molecular Weight
200,261 Da
NCBI Official Full Name
integrin beta-4 isoform X6
NCBI Official Synonym Full Names
integrin beta 4
NCBI Official Symbol
Itgb4 [Similar Products]
NCBI Official Synonym Symbols
CD104; AA407042; C230078O20
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NCBI Protein Information
integrin beta-4
UniProt Protein Name
Integrin beta-4
UniProt Synonym Protein Names
CD_antigen: CD104
UniProt Gene Name
Itgb4 [Similar Products]
UniProt Entry Name
ITB4_MOUSE
NCBI Summary for ITGB4
Integrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the
biology of invasive carcinoma. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for ITGB4
ITGB4 iso2: integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells. Five alternatively spliced isoforms have been described.
Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Receptor, misc.; Cell adhesion
Cellular Component: cell surface; leading edge; integral to membrane; cell cortex; hemidesmosome; membrane; cytoplasm; basal plasma membrane; plasma membrane; basement membrane; integrin complex; cell junction; receptor complex
Molecular Function: G-protein-coupled receptor binding; receptor activity
Biological Process: integrin-mediated signaling pathway; skin development; hemidesmosome assembly; filopodium formation; gut development; multicellular organismal development; cell-matrix adhesion; cell motility involved in cell locomotion; response to wounding; autophagy; cell communication; cell adhesion
Research Articles on ITGB4
1. keratins have a role in controlling beta4-integrin endocytosis involving a plectin-Erk1/2-dependent mechanism relevant for epidermal differentiation and pathogenesis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
