Full Product Name
IYD Polyclonal Antibody
Product Gene Name
anti-IYD antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q6PHW0
Purity/Purification
Affinity purification
Immunogen
Recombinant protein of human IYD
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Other Notes
Small volumes of anti-IYD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-IYD antibody
Polyclonal
Applications Tested/Suitable for anti-IYD antibody
Western Blot (WB)
Application Notes for anti-IYD antibody
WB: 1:200 - 1:2000
Western Blot (WB) of anti-IYD antibody
Western blot analysis of extracts of various cell lines, using IYD antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
NCBI/Uniprot data below describe general gene information for IYD. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001158166.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001164694.1
[Other Products]
UniProt Primary Accession #
Q6PHW0
[Other Products]
UniProt Secondary Accession #
Q2VPW0; Q2VPW1; Q5F1L5; Q5F1L6; Q5THM4; Q6ZP69; Q7Z7D7; Q7Z7D8; C9JFW2[Other Products]
UniProt Related Accession #
Q6PHW0[Other Products]
Molecular Weight
Calculated MW: 33kDa
NCBI Official Full Name
iodotyrosine deiodinase 1 isoform 1
NCBI Official Synonym Full Names
iodotyrosine deiodinase
NCBI Official Symbol
IYD [Similar Products]
NCBI Official Synonym Symbols
TDH4; IYD-1; DEHAL1; C6orf71
[Similar Products]
NCBI Protein Information
iodotyrosine deiodinase 1
UniProt Protein Name
Iodotyrosine deiodinase 1
UniProt Synonym Protein Names
Iodotyrosine dehalogenase 1
Protein Family
Iodotyrosine deiodinase
UniProt Gene Name
IYD [Similar Products]
UniProt Synonym Gene Names
C6orf71; DEHAL1; IYD-1 [Similar Products]
UniProt Entry Name
IYD1_HUMAN
NCBI Summary for IYD
This gene encodes an enzyme that catalyzes the oxidative NADPH-dependent deiodination of mono- and diiodotyrosine, which are the halogenated byproducts of thyroid hormone production. The N-terminus of the protein functions as a membrane anchor. Mutations in this gene cause congenital hypothyroidism due to dyshormonogenesis type 4, which is also referred to as deiodinase deficiency, or iodotyrosine dehalogenase deficiency, or thyroid hormonogenesis type 4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]
UniProt Comments for IYD
IYD: Catalyzes the oxidative NADPH-dependent deiodination of monoiodotyrosine (L-MIT) or diiodotyrosine (L-DIT). Acts during the hydrolysis of thyroglobulin to liberate iodide, which can then reenter the hormone-producing pathways. Acts more efficiently on monoiodotyrosine than on diiodotyrosine. Defects in IYD are the cause of thyroid dyshormonogenesis 4 (TDH4). A disorder due to thyroid dyshormonogenesis, causing severe hypothyroidism, goiter, excessive levels of iodotyrosine in serum and urine, and variable mental deficits derived from unrecognized and untreated hypothyroidism. Belongs to the nitroreductase family. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; EC 1.22.1.1; Oxidoreductase
Chromosomal Location of Human Ortholog: 6q25.1
Cellular Component: integral to membrane; plasma membrane
Molecular Function: oxidoreductase activity; protein binding
Biological Process: thyroid hormone generation
Disease: Thyroid Dyshormonogenesis 4
Research Articles on IYD
1. The rate-limiting processes that contribute to the ability of flavin to promote reductive dehalogenation in human IYD.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
