Full Product Name
IYD siRNA (Human)
Product Synonym Names
C6orf71; DEHAL1; Iodotyrosine dehalogenase 1; IYD-1
Product Gene Name
IYD sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q6PHW0
Specificity
IYD siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human IYD gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of IYD sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
IYD sirna
siRNA to inhibit IYD expression using RNA interference
Applications Tested/Suitable for IYD sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for IYD. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001158166.1
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NCBI GenBank Nucleotide #
NM_001164694.1
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UniProt Primary Accession #
Q6PHW0
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UniProt Secondary Accession #
Q2VPW0; Q2VPW1; Q5F1L5; Q5F1L6; Q5THM4; Q6ZP69; Q7Z7D7; Q7Z7D8; C9JFW2[Other Products]
UniProt Related Accession #
Q6PHW0[Other Products]
Molecular Weight
22,460 Da
NCBI Official Full Name
iodotyrosine dehalogenase 1 isoform 1
NCBI Official Synonym Full Names
iodotyrosine deiodinase
NCBI Official Symbol
IYD [Similar Products]
NCBI Official Synonym Symbols
TDH4; DEHAL1; C6orf71; dJ422F24.1
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NCBI Protein Information
iodotyrosine dehalogenase 1
UniProt Protein Name
Iodotyrosine dehalogenase 1
Protein Family
Iodotyrosine deiodinase
UniProt Gene Name
IYD [Similar Products]
UniProt Synonym Gene Names
C6orf71; DEHAL1; IYD-1 [Similar Products]
UniProt Entry Name
IYD1_HUMAN
NCBI Summary for IYD
This gene encodes an enzyme that catalyzes the oxidative NADPH-dependent deiodination of mono- and diiodotyrosine, which are the halogenated byproducts of thyroid hormone production. The N-terminus of the protein functions as a membrane anchor. Mutations in this gene cause congenital hypothyroidism due to dyshormonogenesis type 4, which is also referred to as deiodinase deficiency, or iodotyrosine dehalogenase deficiency, or thyroid hormonogenesis type 4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]
UniProt Comments for IYD
IYD: Catalyzes the oxidative NADPH-dependent deiodination of monoiodotyrosine (L-MIT) or diiodotyrosine (L-DIT). Acts during the hydrolysis of thyroglobulin to liberate iodide, which can then reenter the hormone-producing pathways. Acts more efficiently on monoiodotyrosine than on diiodotyrosine. Defects in IYD are the cause of thyroid dyshormonogenesis 4 (TDH4). A disorder due to thyroid dyshormonogenesis, causing severe hypothyroidism, goiter, excessive levels of iodotyrosine in serum and urine, and variable mental deficits derived from unrecognized and untreated hypothyroidism. Belongs to the nitroreductase family. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Oxidoreductase; EC 1.22.1.1
Chromosomal Location of Human Ortholog: 6q25.1
Cellular Component: integral to membrane; plasma membrane
Molecular Function: iodide peroxidase activity; oxidoreductase activity
Biological Process: thyroid hormone generation
Disease: Thyroid Dyshormonogenesis 4
Research Articles on IYD
1. A switch between one- and two-electron chemistry of iodotyrosine deiodinase is controlled by substrate.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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