Thymidylate synthase, Recombinant Protein

HST422; TMS; TS; d TMP synthase; EC 2.1.1.45; MGC88736; Thymidylate synthase; Thymidylate synthetase; Tsase; TYMS; TYMS protein; Tyms thymidylate synthetase

For Research Use Only. Not for use in diagnostic procedures.

E Coli

> 95% by SDS-PAGE

Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1 M NaCl.

1 mg/ml (determined by Bradford assay) (lot specific)

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Small volumes of TYMS recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Thymidylate synthase is an intracellular enzyme critical for de novo synthesis of DNA. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. In cancer, expression of this protein is often elevated and becomes further elevated as a result of treatment with the most commonly used chemotherapeutic, 5-fluorouracil (5-Fu). Resistance or lack of response to 5-Fu is attributed to the elevation of thymidylate synthase activity. Recombinant human Thymidylate synthase protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Enzymes & Proteases

SDS-PAGE

37.8 kDa (333aa), confirmed by MALDI-TOF. [Similar Products]

thymidylate synthetase

thymidylate synthase

Thymidylate synthase

TS; TS; TSase  [Similar Products]

Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using, 10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occurring antisense transcript, mitochondrial enolase superfamily member 1 (GeneID:55556), vary inversely when cell-growth progresses from late-log to plateau phase. Polymorphisms in this gene may be associated with etiology of neoplasia, including breast cancer, and response to chemotherapy. [provided by RefSeq, Aug 2017]

Contributes to the de novo mitochondrial thymidylate biosynthesis pathway.

Chiu TH., et al. (2009) Anticancer Res. 29 (11):4503-11. ; Pena MM., et al. (2009) J Biol Chem. 284 (46):31597-607.;

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