Apolipoprotein L1, Polyclonal Antibody

Apo-L; ApoL; Apolipoprotein L-I; ApoL-I; APOL1; APOL

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Antigen Affinity Purified

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Small volumes of anti-APOL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

May play a role in lipid exchange and transport throughout the body. May participate in reverse cholesterol transport from peripheral cells to the liver.

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Recommended dilution: WB:1:500-2000
IHC:1:20-1:200

All lanes: Apolipoprotein L1 antibody at 10ug/ml
Lane 1:A549 whole cell lysate
Lane 2:Hela whole cell lysate
Lane 3:HepG2 whole cell lysate
Secondary
Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 44,46,43 kDa
Observed band size: 44 kDa

Western Blot
Positive WB detected in: hepG2 cell
All lanes: APOL1 antibody at 1.2ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 44,46,43 kDa
Observed band size: 44 kDa

Immunohistochemistry of paraffin-embedded human breast cancer using MBS1497601 at dilution 1:100

Immunohistochemistry of paraffin-embedded human kidney using MBS1497601 at dilution 1:100

42,158 Da

apolipoprotein L1

apolipoprotein L1

Apolipoprotein L1

APOL; Apo-L; ApoL; ApoL-I  [Similar Products]

APOL1_HUMAN

This gene encodes a secreted high density lipoprotein which binds to apolipoprotein A-I. Apolipoprotein A-I is a relatively abundant plasma protein and is the major apoprotein of HDL. It is involved in the formation of most cholesteryl esters in plasma and also promotes efflux of cholesterol from cells. This apolipoprotein L family member may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver. Several different transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008]

APOL1: May play a role in lipid exchange and transport throughout the body. May participate in reverse cholesterol transport from peripheral cells to the liver. Defects in APOL1 are the cause of focal segmental glomerulosclerosis type 4 (FSGS4). It is a renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation. Belongs to the apolipoprotein L family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Channel, chloride; Secreted, signal peptide; Lipid-binding; Secreted

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: extracellular region; extracellular space; intrinsic to membrane

Molecular Function: chloride channel activity; lipid binding; protein binding

Biological Process: cholesterol metabolic process; cytolysis; innate immune response; killing of cells of another organism; lipid transport; lipoprotein metabolic process; receptor-mediated endocytosis

Disease: Focal Segmental Glomerulosclerosis 4, Susceptibility To

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