Apolipoprotein L1, ELISA Kit

Apolipoprotein L1; Apolipoprotein L; Apo-L; ApoL; Apolipoprotein L-I; ApoL-I; APOL1; APOL

For Research Use Only. Not for use in diagnostic procedures.

This assay recognizes recombinant and natural human Apolipoprotein L1. No significant cross-reactivity or interference was observed.

For long term storage, please store the entire kit at -20 degree C.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of APOL1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2885072 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Apolipoprotein L1 (APOL1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing APOL1. The ELISA analytical biochemical technique of the MBS2885072 kit is based on APOL1 antibody-APOL1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect APOL1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, APOL1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human Apolipoprotein L1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein L1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Apolipoprotein L1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Apolipoprotein L1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Apolipoprotein L1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

42,158 Da

apolipoprotein L1

apolipoprotein L1

Apolipoprotein L1

APOL; Apo-L; ApoL; ApoL-I  [Similar Products]

APOL1_HUMAN

This gene encodes a secreted high density lipoprotein which binds to apolipoprotein A-I. Apolipoprotein A-I is a relatively abundant plasma protein and is the major apoprotein of HDL. It is involved in the formation of most cholesteryl esters in plasma and also promotes efflux of cholesterol from cells. This apolipoprotein L family member may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver. Several different transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008]

APOL1: May play a role in lipid exchange and transport throughout the body. May participate in reverse cholesterol transport from peripheral cells to the liver. Defects in APOL1 are the cause of focal segmental glomerulosclerosis type 4 (FSGS4). It is a renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation. Belongs to the apolipoprotein L family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Channel, chloride; Lipid-binding; Secreted

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: extracellular region; extracellular space; intrinsic to membrane

Molecular Function: chloride channel activity; lipid binding; protein binding

Biological Process: cholesterol metabolic process; cytolysis; innate immune response; killing of cells of another organism; lipid transport; lipoprotein metabolic process; receptor-mediated endocytosis

Disease: Focal Segmental Glomerulosclerosis 4, Susceptibility To

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