Thymidylate Synthetase, Polyclonal Antibody

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

The antibody is a rabbit polyclonal antibody raised against TYMS. It has beenselected for its ability to recognize TYMS in immunohistochemical staining andwestern blotting.

Affinity Chromatography

Liquid

200ug/ml (lot specific)

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Small volumes of anti-TYMS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Knock-Out Validation Antibody

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)

Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.

Western Blot: Sample: Human U2OS cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human TYMS Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

Western Blot: Sample: Recombinant protein.

DAB staining on fromalin fixed paraffin- embedded testis tissue)

Knockout Validation: Lane 1: Wild-type U2OS cell lysate; Lane 2: TYMS knockout U2OS cell lysate; Predicted MW: 36,32,26kd Observed MW: 38kd Primary Ab: 1ug/ml Rabbit Anti-Human TYMS Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047)

26,140 Da

thymidylate synthetase

thymidylate synthase

Thymidylate synthase

TS; TS; TSase  [Similar Products]

Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using, 10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occurring antisense transcript, mitochondrial enolase superfamily member 1 (GeneID:55556), vary inversely when cell-growth progresses from late-log to plateau phase. Polymorphisms in this gene may be associated with etiology of neoplasia, including breast cancer, and response to chemotherapy. [provided by RefSeq, Aug 2017]

TYMS: an enzyme in the thymidylate synthase family. Polymorphisms serve as prognostic markers for determining modality of treatment in certain cancers. Genotyping helps predict toxicity to 5-FU-based chemotherapy in patients with colorectal cancer and the response to platinum-based chemotherapy in advanced NSCLC. A common polymorphism is a determinant of red blood cell folate and homocysteine concentrations. Homodimer.

Protein type: Cofactor and Vitamin Metabolism - one carbon pool by folate; DNA repair, damage; EC 2.1.1.45; Methyltransferase; Mitochondrial; Nucleotide Metabolism - pyrimidine

Chromosomal Location of Human Ortholog: 18p11.32

Cellular Component: cytoplasm; cytosol; mitochondrial inner membrane; mitochondrial matrix; mitochondrion; nucleolus; nucleus

Molecular Function: cofactor binding; drug binding; folic acid binding; nucleotide binding; protein homodimerization activity; thymidylate synthase activity; translation repressor activity, nucleic acid binding

Biological Process: aging; cartilage development; cell growth; cell proliferation; circadian rhythm; developmental growth; dTMP biosynthetic process; dTTP biosynthetic process; dUMP metabolic process; G1/S-specific transcription in mitotic cell cycle; immortalization of host cell by virus; methylation; negative regulation of translation; nucleobase, nucleoside and nucleotide interconversion; response to cytokine; response to drug; response to ethanol; response to folic acid; response to glucocorticoid stimulus; response to organophosphorus; response to progesterone; response to toxin; response to vitamin A; uracil metabolic process

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