Product Name
RPS17, siRNA
Full Product Name
RPS17 siRNA (Human)
Product Synonym Names
40S ribosomal protein S17
Product Gene Name
RPS17 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P08708
Specificity
RPS17 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RPS17 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of RPS17 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
RPS17 sirna
siRNA to inhibit RPS17 expression using RNA interference
Applications Tested/Suitable for RPS17 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for RPS17. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001012.1
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NCBI GenBank Nucleotide #
NM_001021.4
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UniProt Primary Accession #
P08708
[Other Products]
UniProt Secondary Accession #
B2R4U4[Other Products]
UniProt Related Accession #
P08708[Other Products]
Molecular Weight
15,550 Da
NCBI Official Full Name
40S ribosomal protein S17
NCBI Official Synonym Full Names
ribosomal protein S17
NCBI Official Symbol
RPS17 [Similar Products]
NCBI Official Synonym Symbols
S17; DBA4; RPS17L; RPS17L1; RPS17L2
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NCBI Protein Information
40S ribosomal protein S17
UniProt Protein Name
40S ribosomal protein S17
Protein Family
40S ribosomal protein
UniProt Gene Name
RPS17 [Similar Products]
UniProt Entry Name
RS17_HUMAN
NCBI Summary for RPS17
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S17E family of ribosomal proteins and is located in the cytoplasm. Mutations in this gene cause Diamond-Blackfan anemia 4. Alternative splicing of this gene results in multiple transcript variants. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Apr 2014]
UniProt Comments for RPS17
RPS17: Defects in RPS17 are the cause of Diamond-Blackfan anemia type 4 (DBA4). DBA4 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies. Belongs to the ribosomal protein S17e family.
Protein type: Translation; Ribosomal
Chromosomal Location of Human Ortholog: 15q
Cellular Component: focal adhesion; membrane; ribosome; cytosol
Molecular Function: structural constituent of ribosome
Biological Process: SRP-dependent cotranslational protein targeting to membrane; viral reproduction; translation; translational termination; viral infectious cycle; erythrocyte homeostasis; ribosomal small subunit biogenesis and assembly; translational elongation; cellular protein metabolic process; translational initiation; mRNA catabolic process, nonsense-mediated decay; ribosomal small subunit assembly and maintenance; gene expression; viral transcription; rRNA processing
Disease: Diamond-blackfan Anemia 4
Research Articles on RPS17
1. Human S17 insertion was a major factor for hepatitis E virus in cell culture adaptation.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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