Cholinergic Receptor, Nicotinic, Alpha 4 (CHRNa4), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 20; NC_000020.10 (61974662..61992748, complement). Location: 20q13.2-q13.3

Store all reagents at 2-8 degree C

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Small volumes of CHRNa4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9311790 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cholinergic Receptor, Nicotinic, Alpha 4 (CHRNa4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CHRNa4. The ELISA analytical biochemical technique of the MBS9311790 kit is based on CHRNa4 antibody-CHRNa4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CHRNa4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CHRNa4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

69,957 Da

cholinergic receptor, nicotinic, alpha 4 (neuronal)

neuronal acetylcholine receptor subunit alpha-4; cholinergic receptor, nicotinic, alpha polypeptide 4; neuronal nicotinic acetylcholine receptor alpha-4 subunit

Neuronal acetylcholine receptor subunit alpha-4

NACRA4  [Similar Products]

ACHA4_HUMAN

This gene encodes a nicotinic acetylcholine receptor, which belongs to a superfamily of ligand-gated ion channels that play a role in fast signal transmission at synapses. These pentameric receptors can bind acetylcholine, which causes an extensive change in conformation that leads to the opening of an ion-conducting channel across the plasma membrane. This protein is an integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor. Mutations in this gene cause nocturnal frontal lobe epilepsy type 1. Polymorphisms in this gene that provide protection against nicotine addiction have been described. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]

nAChRA4: a ligand-gated ion channels that binds acetylcholine and mediates fast signal transmission at synapses. After binding acetylcholine, these pentameric receptors undergo an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. An integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor.

Protein type: Channel, ligand-gated; Receptor, misc.; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 20q13.33

Cellular Component: nicotinic acetylcholine-gated receptor-channel complex; postsynaptic membrane; membrane; cell soma; dendrite; plasma membrane; integral to membrane; cell junction; external side of plasma membrane

Molecular Function: acetylcholine receptor activity; acetylcholine binding; nicotinic acetylcholine-activated cation-selective channel activity; ligand-gated ion channel activity

Biological Process: response to nicotine; regulation of action potential; B cell activation; behavioral response to nicotine; locomotory behavior; respiratory gaseous exchange; DNA repair; sensory perception of pain; signal transduction; regulation of dopamine secretion; synaptic transmission, cholinergic; neurological system process; regulation of inhibitory postsynaptic membrane potential; membrane depolarization; synaptic transmission; regulation of membrane potential; calcium ion transport; response to hypoxia; ion transport; response to oxidative stress; cognition

Disease: Epilepsy, Nocturnal Frontal Lobe, 1; Tobacco Addiction, Susceptibility To

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