Product Name
PVRL4, siRNA
Full Product Name
PVRL4 siRNA (Human)
Product Synonym Names
LNIR; PRR4; Poliovirus receptor-related protein 4; Ig superfamily receptor LNIR; Nectin-4
Product Gene Name
PVRL4 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q96NY8
Specificity
PVRL4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PVRL4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of PVRL4 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
PVRL4 sirna
siRNA to inhibit PVRL4 expression using RNA interference
Applications Tested/Suitable for PVRL4 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for PVRL4. It may not necessarily be applicable to this product.
NCBI Accession #
NP_112178.2
[Other Products]
NCBI GenBank Nucleotide #
NM_030916.2
[Other Products]
UniProt Primary Accession #
Q96NY8
[Other Products]
UniProt Secondary Accession #
Q96K15; B4DQW3[Other Products]
UniProt Related Accession #
Q96NY8[Other Products]
Molecular Weight
24,341 Da
NCBI Official Full Name
nectin-4
NCBI Official Synonym Full Names
poliovirus receptor-related 4
NCBI Official Symbol
PVRL4 [Similar Products]
NCBI Official Synonym Symbols
LNIR; PRR4; EDSS1; nectin-4
[Similar Products]
NCBI Protein Information
nectin-4
UniProt Protein Name
Nectin-4
UniProt Synonym Protein Names
Ig superfamily receptor LNIR; Poliovirus receptor-related protein 4
UniProt Gene Name
PVRL4 [Similar Products]
UniProt Synonym Gene Names
LNIR; PRR4 [Similar Products]
UniProt Entry Name
PVRL4_HUMAN
NCBI Summary for PVRL4
This gene encodes a member of the nectin family. The encoded protein contains two immunoglobulin-like (Ig-like) C2-type domains and one Ig-like V-type domain. It is involved in cell adhesion through trans-homophilic and -heterophilic interactions. It is a single-pass type I membrane protein. The soluble form is produced by proteolytic cleavage at the cell surface by the metalloproteinase ADAM17/TACE. The secreted form is found in both breast tumor cell lines and breast tumor patients. Mutations in this gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder. Alternatively spliced transcript variants have been found but the full-length nature of the variant has not been determined.[provided by RefSeq, Jan 2011]
UniProt Comments for PVRL4
nectin 4: Seems to be involved in cell adhesion through trans- homophilic and -heterophilic interactions, the latter including specifically interactions with PVRL2/nectin-1. Does not act as receptor for alpha-herpesvirus entry into cells. Defects in PVRL4 are the cause of ectodermal dysplasia- syndactyly syndrome type 1 (EDSS1). EDSS1 is a form of ectodermal dysplasia, a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDSS1 is characterized by the association of hair and teeth abnormalities with cutaneous syndactyly of the hands and/or feet. Hair morphologic abnormalities include twists at irregular intervals (pilli torti) and swelling along the shafts, particularly associated with areas of breakage. Dental findings consist of abnormally widely spaced teeth, with peg-shaped and conical crowns. Patients have normal sweating. Belongs to the nectin family.
Protein type: Membrane protein, integral; Cell adhesion
Chromosomal Location of Human Ortholog: 1q23.3
Cellular Component: adherens junction; integral to membrane; plasma membrane
Molecular Function: protein binding
Biological Process: intercellular junction assembly and maintenance; viral reproduction; cell adhesion
Disease: Ectodermal Dysplasia-syndactyly Syndrome 1
Research Articles on PVRL4
1. T, p.Asp61*) in the PVRL4 gene.">The present study described clinical investigation of the EDSS1 identified in a large consanguineous family; DNA sequence analysis revealed a novel homozygous nonsense mutation (181C>T, p.Asp61*) in the PVRL4 gene.
Precautions
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Disclaimer
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