Histamine, ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Human HIS. No significant cross-reactivity or interference between Human HIS and analogues was observed.

Store at 2-8 degree C.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of HIS elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS285080 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Histamine, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HIS. The ELISA analytical biochemical technique of the MBS285080 kit is based on HIS antibody-HIS antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HIS antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HIS. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Aldose reductase (AR) is an NADPH-dependent enzyme that was first identified by its ability to reduce glucose to sorbitol. The deduced 316-amino acid AKR1B10 protein shares 71% amino acid identity with AR. Northern blot analysis revealed that AKR1B10 is overexpressed in 54% of HCCs, while AR is overexpressed in 29% of HCCs. Northern blot analysis of normal tissues showed that unlike the ubiquitously expressed AR, AKR1B10 is expressed most abundantly in small intestine and colon, with lower levels in liver, thymus, prostate, testis, and skeletal muscle. AR and ARL1 reduce a similar spectrum of aromatic and aliphatic aldehyde substrates.Highest expression of AKR1B10 in adrenal gland, with modest expression in stomach, placenta, small intestine, and pancreas, and lower expression in all other tissues tested.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate HIS in samples. An antibody specific for HIS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHIS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HIS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HIS bound in the initial step. The color development is stopped and the intensity of the color is measured.

44,541 Da

histamine receptor H2

histamine H2 receptor

Histamine H2 receptor

H2R; HH2R  [Similar Products]

HRH2_HUMAN

Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. Histamine receptor H2 belongs to the family 1 of G protein-coupled receptors. It is an integral membrane protein and stimulates gastric acid secretion. It also regulates gastrointestinal motility and intestinal secretion and is thought to be involved in regulating cell growth and differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

H2R: The H2 subclass of histamine receptors mediates gastric acid secretion. Also appears to regulate gastrointestinal motility and intestinal secretion. Possible role in regulating cell growth and differentiation. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and, through a separate G protein-dependent mechanism, the phosphoinositide/protein kinase (PKC) signaling pathway. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q35.2

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: histamine receptor activity

Biological Process: G-protein signaling, coupled to cyclic nucleotide second messenger; gastrin-induced gastric acid secretion; gland development; gut development; histamine-induced gastric acid secretion; immune response; memory; positive regulation of vasoconstriction; regulation of synaptic plasticity; visual learning

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